Isobaric labels for mass spectrometric analysis of peptides and method thereof
First Claim
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1. A method for mass spectrometric analysis of isobarically labeled reactive peptides comprising:
- a) providing two or more samples, each sample comprising one or more reactive peptide analytes,b) reacting a first sample of the two or more samples with a first labeling reagent of a set of isobaric labeling reagents,c) reacting a second sample of the two or more samples with a second labeling reagent of the set of isobaric labeling reagents, the second labeling reagent differing isobarically from the first labeling reagent, wherein steps b) and c) thereby produce two or more differentially labeled samples each comprising one or more isobarically labeled peptide analytes,d) mixing together the two or more differentially labeled samples to form a sample mixture comprising two or more isobarically labeled peptide analytes of the formula;
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Abstract
This invention pertains to methods, mixtures, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes.
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Citations
9 Claims
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1. A method for mass spectrometric analysis of isobarically labeled reactive peptides comprising:
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a) providing two or more samples, each sample comprising one or more reactive peptide analytes, b) reacting a first sample of the two or more samples with a first labeling reagent of a set of isobaric labeling reagents, c) reacting a second sample of the two or more samples with a second labeling reagent of the set of isobaric labeling reagents, the second labeling reagent differing isobarically from the first labeling reagent, wherein steps b) and c) thereby produce two or more differentially labeled samples each comprising one or more isobarically labeled peptide analytes, d) mixing together the two or more differentially labeled samples to form a sample mixture comprising two or more isobarically labeled peptide analytes of the formula;
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2. A method for mass spectrometric analysis of isobarically labeled reactive peptides comprising:
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a) providing two or more samples, each sample comprising one or more reactive peptide analytes, b) reacting a first sample of the two or more samples with a first labeling reagent of a set of isobaric labeling reagents, c) reacting a second sample of the two or more samples with a second labeling reagent of the set of isobaric labeling reagents, the second labeling reagent differing isobarically from the first labeling reagent, wherein steps b) and c) produce two or more differentially labeled samples each comprising one or more isobarically labeled peptide analytes, d) mixing together the two or more differentially labeled samples to form a sample mixture comprising two or more isobarically labeled peptide analytes of the formula;
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3. A method for mass spectrometric analysis of isobarically labeled reactive peptides comprising:
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a) reacting providing two or more samples, each sample comprising one or more reactive peptide analytes, b) reacting a first sample of the two or more samples with a first labeling reagent of a set of isobaric labeling reagents, c) reacting a second sample of the two or more samples with a second labeling reagent of the set of isobaric labeling reagents, the second labeling reagent differing isobarically from the first labeling reagent, wherein steps b) and c) produce two or more differentially labeled samples each comprising one or more isobarically labeled peptide analytes d) mixing together the two or more differentially labeled samples to form a sample mixture comprising two or more isobarically labeled peptide analytes of the formula;
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4. A method for mass spectrometric analysis of isobarically labeled reactive peptides comprising:
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a) providing two or more samples, each sample comprising one or more reactive peptide analytes, b) reacting a first sample of the two or more samples with a first labeling reagent of a set of isobaric labeling reagents, c) reacting a second sample of the two or more samples with a second labeling reagent of the set of isobaric labeling reagents, the second labeling reagent differing isobarically from the first labeling reagent, wherein steps b) and c) produce two or more differentially labeled samples each comprising one or more isobarically labeled peptide analytes, d) mixing together the two or more differentially labeled samples to form a sample mixture comprising two or more isobarically labeled peptide analytes of the formula;
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- 5. A mixture comprising at least two labeled peptide analytes, wherein each of the two labeled peptide analytes originates from a different sample combined to form the mixture and each comprises a different isobaric label from a same set of isobaric labels, each isobaric label comprising a 5, 6, or 7 membered heterocyclic ring comprising a ring nitrogen atom that is N-alkylated with a substituted or unsubstituted acetic acid moiety to which the peptide analyte is linked through the carbonyl carbon of the N-alkyl acetic acid moiety, wherein each different isobaric label comprises one or more heavy atom isotopes.
Specification