Methods for stabilizing protein solutions
First Claim
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1. A method for suppressing cryoprecipitation of IgM in a solution at a temperature of 1°
- C. to 7°
C., wherein the method comprises adding a citric acid buffer to a first solution comprising the IgM to form a second solution comprising the IgM at a concentration of 20 mg/ml or greater, and maintaining the second solution at 1°
C. to 7°
C., thereby suppressing cryoprecipitation of the IgM.
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Abstract
The present inventors examined use of citric acid buffers for suppressing cryoprecipitation of IgM at a pH range and salt concentration suitable for storing IgM. As a result, the present inventors discovered that citric acid buffers significantly suppress cryoprecipitation.
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Citations
18 Claims
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1. A method for suppressing cryoprecipitation of IgM in a solution at a temperature of 1°
- C. to 7°
C., wherein the method comprises adding a citric acid buffer to a first solution comprising the IgM to form a second solution comprising the IgM at a concentration of 20 mg/ml or greater, and maintaining the second solution at 1°
C. to 7°
C., thereby suppressing cryoprecipitation of the IgM. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
- C. to 7°
Specification