Method for detecting the presence or absence of a gas bubble in an aqueous liquid

• US 7,807,041 B2
• Filed: 08/31/2005
• Issued: 10/05/2010
• Est. Priority Date: 09/02/2004
• Status: Expired due to Fees

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: Grubler, Robert, Landschutzer, Egon, Kaltenbeck, Heinz
• Primary Examiner(s): Nguyen; Nam X
• Assistant Examiner(s): Kaur; Gurpreet
• Application Number:
  US11/216,743
• Publication Number:
  US 20060042963A1
• Time in Patent Office: 1,861 Days
• Field of Search: 205/775, 205/779, 205/785.5, 205/792, 205/783, 205/782, 204/409, 204/431, 204/432, 204400-435
• US Class Current: 205/775
• CPC Class Codes: G01N 27/404 Cells with anode, cathode and cell electrolyte on the same side of a permeable membrane which separates them from the sample fluid , e.g. Clark-type oxygen sensors
• CPC Class Codes: G01N 27/4163 checking the operation of, or calibrating, the measuring apparatus G01N27/3274, G01N27/4175 and G01N33/0006 take precedence

Abstract

A method for detecting the presence or absence of a gas bubble in an aqueous liquid is provided comprising providing an amperometric sensor positioned within a measuring chamber, wherein the amperometric sensor is configured to determine the concentration of a gaseous component dissolved in a liquid, the amperometric sensor comprising a sensitive region; positioning the liquid in the measuring chamber; taking at least one first measurement value of the gaseous component from a first portion of the liquid after a predetermined response time, the first portion located in the sensitive region of the sensor; moving the liquid along in the measuring chamber, such that a second portion of the liquid, which had previously been located outside the sensitive region of the amperometric sensor, is positioned in the sensitive region; taking at least one second measurement value of the gaseous component from the second portion of the liquid; and detecting the presence or absence of a gas bubble by comparing the first and second measured values. The gas bubble, if present, is in at least partial contact with the sensitive region of the amperometric sensor.

Claims

1. A method for detecting the presence or absence of a gas bubble in an aqueous liquid, compromising:

providing an amperometric sensor positioned within a measuring chamber, wherein said amperometric sensor is configured to determine the concentration of a gaseous component dissolved in said liquid, said amperometric sensor comprising a sensitive region;

positioning said liquid in said measuring chamber;

using said amperometric sensor to take at least one first measurement value of said gaseous component from a first portion of said liquid after a predetermined response time, said first portion located in said sensitive region of said sensor;

moving said liquid along in said measuring chamber without significant pressure change in said liquid, such that a second portion of said liquid within said measuring chamber, which had previously been located outside said sensitive region of said amperometric sensor, is positioned in said sensitive region;

using said amperometric sensor to take at least one second measurement value of said gaseous component from said second portion of said liquid; and

detecting the presence or absence of a gas bubble by comparing said first and second measured values, wherein said liquid is free of bubbles in said sensitive region of said amperometric sensor, if said second measured value is higher by a predetermined amount than said first measured value.

2. The method of claim 1, wherein said gas bubble is in at least partial contact with said sensitive region of said amperometric sensor.

3. The method of claim 1 wherein:

providing an amperometric sensor positioned within a measuring chamber comprises providing said amperometric sensor positioned within a measuring chamber with an open inlet and an open outlet; and

moving said liquid along in said measuring chamber, without significant pressure change in said liquid, comprises;

moving said second portion of said liquid to said sensitive region of said sensor in said measuring chamber with said open inlet and said open outlet without significant change in pressure.

4. The method of claim 3, wherein said liquid is moved by a pumping or suction device.

5. The method of claim 1, wherein said gas bubble comprises air.

6. The method of claim 1, wherein said amperometric sensor is a gas sensor.

7. The method of claim 6, wherein said gas sensor is an O₂ sensor.

8. The method of claim 1, wherein said aqueous liquid comprises a fluid selected from calibrating fluid, control fluid or sample fluid.

9. The method of claim 8, wherein said sample fluid is a biological fluid.

10. The method of claim 9, wherein said biological fluid is blood.

11. A method for detecting the presence or absence of a gas bubble in an aqueous liquid, compromising:

providing an amperometric sensor positioned within a measuring chamber, wherein said amperometric sensor is configured to determine the concentration of a gaseous component dissolved in said liquid, said amperometric sensor comprising a sensitive region;

positioning said liquid in said measuring chamber such that a first portion of said liquid is located in said sensitive region of said sensor;

using said amperometric sensor to take at least one first measurement value of said gaseous component from said first portion of said liquid after a predetermined response time;

moving said liquid along in said measuring chamber such that;

a second portion of said liquid within said measuring chamber, which had previously been located outside said sensitive region of said amperometric sensor is positioned in said sensitive region, and

a bubble, if present within said sensitive region of said sensor when taking said at least one first measurement value, does not move from said sensitive region in response to moving said liquid;

using said amperometric sensor to take at least one second measurement value of said gaseous component from said second portion of said liquid; and

detecting the presence or absence of a gas bubble by comparing said first and second measured values, wherein said liquid is free of bubbles in said sensitive region of said amperometric sensor, if said second measured value is higher by a predetermined amount than said first measured value.

12. The method of claim 11, wherein providing an amperometric sensor positioned within a measuring chamber further comprises:

providing said amperometric sensor positioned within a measuring chamber with an open inlet and an open outlet.

13. The method of claim 12, wherein moving said liquid along in said measuring chamber, comprises:

moving said second portion of said liquid to said sensitive region of said sensor in said measuring chamber with said open inlet and said open outlet without significant change in pressure.

14. The method of claim 11, wherein said liquid comprises a fluid selected from calibrating fluid, control fluid or sample fluid.

15. The method of claim 14 wherein said sample fluid is a biological fluid.

16. The method of claim 11, wherein detecting the presence or absence of a gas bubble by comparing said first and second measured values comprises:

determining that a bubble is present if said second measured value does not rise compared to said first measured value; and

determining that a bubble is not present if said second measured value rises compared said first measured value.

17. A system to detect the presence or absence of a gas bubble in an aqueous liquid, compromising:

an amperometric sensor positioned within a measuring chamber having an open inlet and an open outlet, wherein said amperometric sensor is configured to determine the concentration of a gaseous component dissolved in said liquid, said amperometric sensor comprising a sensitive region, wherein;

said measuring chamber is provided for receiving said liquid; and

said amperometric sensor is provided for taking at least one first measurement value of said gaseous component from a first portion of said liquid after a predetermined response time, said first portion located in said sensitive region of said sensor; and

at least one of a pump and a suction device, to move said liquid along in said measuring chamber without significant pressure change in said liquid, such that a second portion of said liquid within said measuring chamber, which had previously been located outside said sensitive region of said amperometric sensor, is positioned in said sensitive region;

wherein;

said amperometric sensor takes at least one second measurement value of said gaseous component from said second portion of said liquid such that the presence or absence of a gas bubble is detected by comparing said first and second measured values, wherein said liquid is free of bubbles in said sensitive region of said amperometric sensor, if said second measured value is higher by a predetermined amount than said first measured value.

18. The system according to claim 17, wherein said at least one of a pump and a suction device, moves said liquid without causing a significant pressure change in said liquid so that a bubble, if present within said sensitive region of said sensor remains adhered to said sensitive region after moving said liquid.