Methods for identification of merle gene

• US 7,807,370 B2
• Filed: 08/16/2006
• Issued: 10/05/2010
• Est. Priority Date: 08/16/2005
• Status: Active Grant

• Current Assignee: Merlogen LLC
• Original Assignee: Merlogen LLC
• Inventors: Rees, Christine, Wahl, Jacquelyn, Clark, Leigh Anne, Murphy, Keith E.
• Primary Examiner(s): WOOLWINE, SAMUEL C
• Application Number:
  US12/063,964
• Publication Number:
  US 20080227102A1
• Time in Patent Office: 1,511 Days
• Field of Search: None
• US Class Current: 435/6.12
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 2600/124 Animal traits, i.e. production traits, including athletic performance or the like
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers
• CPC Class Codes: C12Q 2600/16 Primer sets for multiplex assays

Abstract

Animals with mutations in SILV present with the merle coat color pattern phenotype. Methods for the identification of animals that harbor a mutation in the SILV gene are described. Mutations in the SILV gene can be identified from any biological sample such as a cell or tissue that contains genomic DNA. A microsatellite marker identified using linkage disequilibrium mapping that segregates with merle is also described.

Claims

1. A method for identifying a merle and/or a cryptic merle allele in a canine animal, the method comprising:

analyzing a biological sample obtained from the canine animal for mutations in the SILV gene, wherein the presence of at least one insertion mutation at the boundary of intron 10 and exon 11 of the SILV gene indicates the canine animal has a merle or a cryptic merle allele, wherein the insertion comprises a short interspersed nucleotide element (SINE).

2. The method of claim 1, wherein the insertion is present between nucleotide positions corresponding to positions 43 and 44 of SEQ ID NO:

24.

3. The method of claim 1, wherein the insertion is flanked by the first 15 nucleotides of any one of SEQ ID NOs:

26-36.

4. The method of claim 1, wherein the merle allele is cryptic if the length of the poly-A segment of the SINE element is reduced by about 30 nucleotides relative to the length of any of the poly-A segments corresponding to the poly-T segments of the SINE elements shown in SEQ ID NOs:

26-33.

5. The method of claim 1, wherein the canine animal is a dog.

6. The method of claim 1, wherein the canine animal has a merle allele.

7. The method of claim 6, wherein the merle allele comprises any one of SEQ ID NOs:

26-33.

8. The method of claim 1, wherein the canine animal has a cryptic merle allele.

9. The method of claim 8, wherein the cryptic merle allele is SEQ ID NO:

34, 35 or 36.

10. The method of claim 1, wherein the biological sample is cell or tissue containing genomic DNA obtained from a dog.

11. The method of claim 1, wherein the biological sample is analyzed by performing PCR using genomic DNA as a template.