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Method of optically imaging biological tissues by using fluorescence, in particular for defining regions of interest in tissues to be analyzed by tomography

  • US 7,809,431 B2
  • Filed: 04/17/2007
  • Issued: 10/05/2010
  • Est. Priority Date: 04/24/2006
  • Status: Active Grant
First Claim
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1. A method of optically imaging at least one biological tissue, the method comprising:

  • a) introducing at least one fluorescent marker into said at least one tissue;

    b) exciting said at least one marker by incident light radiations and detecting bands of emission relating to fluorescence emitted by said at least one marker in response to that excitation; and

    c) analyzing intensities of fluorescence relative to said emission bands,wherein the step b) includes,sequentially exciting said at least one marker at n different incident excitation wavelengths λ

    i, said at least one marker excitable by at least two of these n wavelengths λ

    i and to emit in response to each wavelength λ

    i a series Si of m simultaneous emission bands Bj having m different maximum wavelengths λ



    j that are substantially same from one series Si to another (where n and m are independent integers equal to or greater than 2 and where i and j respectively vary from 1 to n and from 1 to m), anddetecting at least two of the series Si that each comprise the m bands Bj emitted simultaneously in order to deduce therefrom in the step c) at least one of an estimate of a three-dimensional location of said marker in the at least one tissue and mean absorption coefficients of the at least one tissue for the excitation wavelengths λ

    i.

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