Polyallylamine conjugates and applications thereof for biological signal amplification
First Claim
1. A polyallylamine conjugate, consisting of polyallylamine, a signal molecule, and a capture agent, wherein said signal molecule is a fluorescent molecule which is cy5;
- said capture agent is a modified antibody linked to a cross-linking agent, wherein the antibody is modified by a compound having functional groups selected from the group consisting of —
SH, —
CO, —
NH2 and —
N═
C═
S, and a cross-linking agent selected from the group consisting of N-succinimidyl 3-(2-pyridyldithio) propionate, glutaraldehyde, succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC), maleimide, iodoacetamide, protected thiol and sulfosuccinimidyl-6-(3′
-[2-pyridyldithio] propionamido) hexanoate (sulfo-LC-SPDP), and wherein said cross-linking agent assists the binding of said capture agent with the —
NH2 functional groups of polyallylamine; and
the signal molecule and capture agent is covalently bound to the polyallylamine via —
NH2 side chain of the polyallylamine.
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Accused Products
Abstract
Polyallylamine conjugates and applications thereof for biological signal amplification are provided by utilizing the essential amino group of polyallylamine to covalently bind with capture agents and signal molecules having the functional groups selected from a group consisting of —NHS, —CO, —S═O2 and —C═O—C═O. The resulting conjugates having more than one signaling entities can be further implemented for biological expression with enhancing effect on biological signal intensity, such that the sensitivity of detection for the variation between biological interactions is largely increased.
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Citations
15 Claims
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1. A polyallylamine conjugate, consisting of polyallylamine, a signal molecule, and a capture agent, wherein said signal molecule is a fluorescent molecule which is cy5;
- said capture agent is a modified antibody linked to a cross-linking agent, wherein the antibody is modified by a compound having functional groups selected from the group consisting of —
SH, —
CO, —
NH2 and —
N═
C═
S, and a cross-linking agent selected from the group consisting of N-succinimidyl 3-(2-pyridyldithio) propionate, glutaraldehyde, succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC), maleimide, iodoacetamide, protected thiol and sulfosuccinimidyl-6-(3′
-[2-pyridyldithio] propionamido) hexanoate (sulfo-LC-SPDP), and wherein said cross-linking agent assists the binding of said capture agent with the —
NH2 functional groups of polyallylamine; and
the signal molecule and capture agent is covalently bound to the polyallylamine via —
NH2 side chain of the polyallylamine. - View Dependent Claims (3, 4, 5, 7, 8, 9)
- said capture agent is a modified antibody linked to a cross-linking agent, wherein the antibody is modified by a compound having functional groups selected from the group consisting of —
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2. A polyallylamine conjugate, consisting essentially of polyallylamine, a signal molecule, and a capture agent, wherein said signal molecule is a fluorescent molecule which is cy5;
- said capture agent is a modified antibody linked to a cross-linking agent, wherein the antibody is modified by a compound having functional groups selected from the group consisting of —
SH, —
CO, —
NH2 and —
N═
C═
S, and a cross-linking agent selected from the group consisting of N-succinimidyl 3-(2-pyridyldithio) propionate, glutaraldehyde, succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC), maleimide, iodoacetamide, protected thiol and sulfosuccinimidyl-6-(3′
-[2-pyridyldithio] propionamido) hexanoate (sulfo-LC-SPDP), and wherein said cross-linking agent assists the binding of said capture agent with the —
NH2 functional groups of polyallylamine; and
the signal molecule and capture agent is covalently bound to the polyallylamine via —
NH2 side chain of the polyallylamine. - View Dependent Claims (6, 10, 11, 12, 13, 14, 15)
- said capture agent is a modified antibody linked to a cross-linking agent, wherein the antibody is modified by a compound having functional groups selected from the group consisting of —
Specification