Measuring probe and production process thereof

• US 7,811,829 B2
• Filed: 06/07/2007
• Issued: 10/12/2010
• Est. Priority Date: 06/08/2006
• Status: Expired due to Fees

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Suzuki, Hiroshi, Imamura, Takeshi, Kikuchi, Yoshihiko
• Primary Examiner(s): BROWN, MELANIE YU
• Application Number:
  US11/808,139
• Publication Number:
  US 20080000308A1
• Time in Patent Office: 1,223 Days
• Field of Search: 435/7.1, 435/283.1, 435/287.1, 435/287.2, 436/518, 436/528, 436/530, 436/532
• US Class Current: 436/518
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

To provide a measuring probe excellent in sensitivity and reproducibility, simple operation in the protein immobilization onto a support can prevent peeling or damage. A layer containing a protein is adsorbed onto a support surface to fully encircle the long axis of the support, and the adsorbed layer is treated for cross-linking with a cross-linking agent in a concentration of 1 to 5 moles to 1 mole of the protein.

Claims

1. A measuring probe for detecting a specific analyte in a sample in contact with the sample, the probe comprising a support and a layer of a protein,

wherein the support has a fiber-shaped portion, a flange portion, and a convex portion,

wherein the protein layer has a circular cross-section so as to fully encircle the fiber-shaped portion,

wherein the protein in the protein layer has been cross-linked by using a solution containing a cross-linking agent in a concentration 1 to 5 times as much as that of the protein, and

wherein the protein layer is formed at the fiber-shaped portion.

2. The measuring probe according to claim 1, wherein the protein layer is formed to have a portion fully encircling an axis of elongation of the fiber-shaped portion.

3. The measuring probe according to claim 1, wherein the cross-linking agent is a bifunctional cross-linking agent, and the functional group for the cross-linking is selected from the group consisting of aldehyde, carboxylic acid and carboxylic acid ester.

4. The measuring probe according to claim 3, wherein the cross-linking agent is glutaraldehyde.

5. The measuring probe according to claim 1, wherein the protein contained in the protein layer is a component for detecting the analyte, the component being an enzyme or a receptor.

6. The measuring probe according to claim 1, wherein the protein contained in the protein layer is a component for detecting the analyte, the component being an antibody or an antigen or a fragment thereof.

7. The measuring probe according to claim 1, wherein a component for detecting the analyte is held on a subbing layer being the protein layer.

8. The measuring probe according to claim 7, wherein the detecting component is selected from the group consisting of antibody, antigen, enzyme, receptor, nucleobase, sugar chain and metal coordinate.

9. The measuring probe according to claim 1, wherein the support has a configuration selected from the group consisting of fiber-, brush- and mesh-like configurations.

10. The measuring probe according to claim 1, wherein the support is an optical waveguide.

11. The measuring probe according to claim 10, wherein the optical waveguide has, at an end thereof, an input/output section for optical signals.

12. The measuring probe according to claim 1, wherein the protein has been cross-linked by dipping only the fiber-shaped portion in the solution.

13. The measuring probe according to claim 1, wherein the convex portion is constructed to receive and emit light.

14. The measuring probe according to claim 1, wherein the molar ratio of the cross-linking agent to the protein is about 1:

1 after the protein has been cross-linked.

15. The measuring probe according to claim 1, wherein the protein layer comprises a cross-linked polymer that provides space around the protein.