Methods and materials for making and using transgenic dicamba-degrading organisms

• US 7,812,224 B2
• Filed: 12/27/2002
• Issued: 10/12/2010
• Est. Priority Date: 04/04/1997
• Status: Expired due to Fees

• Current Assignee: Board of Regents of the University of Nebraska
• Original Assignee: Board of Regents of the University of Nebraska
• Inventors: Wang, Xiao-Zhuo, Herman, Patricia L., Weeks, Donald P.
• Primary Examiner(s): Kruse; David H
• Application Number:
  US10/330,662
• Publication Number:
  US 20030115626A1
• Time in Patent Office: 2,846 Days
• Field of Search: 800/300, 800/288, 435/320.1, 435/419, 435/252.3, 536/23.2, 536/23.7
• US Class Current: 800/300
• CPC Class Codes: C07K 14/79 Transferrins, e.g. lactoferrins, ovotransferrins
• CPC Class Codes: C12N 15/8209 Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
• CPC Class Codes: C12N 15/8259 Phytoremediation
• CPC Class Codes: C12N 15/8274 for herbicide resistance
• CPC Class Codes: C12N 9/00 Enzymes; Proenzymes; Compositions thereof preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113; Processes for preparing, activating, inhibiting, separating or purifying enzymes preparation of malt C12C1/00
• CPC Class Codes: C12N 9/0004 Oxidoreductases (1.)
• CPC Class Codes: C12N 9/0036 acting on NADH or NADPH (1.6)
• CPC Class Codes: C12N 9/0069 acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
• CPC Class Codes: C12N 9/1007 Methyltransferases (general) (2.1.1.)

Abstract

The invention provides isolated and at least partially-purified dicamba-degrading enzymes, isolated DNA molecules coding for dicamba-degrading enzymes, DNA constructs coding for dicamba-degrading enzymes, transgenic host cells comprising DNA coding for dicamba-degrading enzymes, and transgenic plants and plant parts comprising one or more cells comprising DNA coding for dicamba-degrading enzymes. Expression of the dicamba-degrading enzymes results in the production of dicamba-degrading organisms, including dicamba-tolerant plants. The invention further provides a method of controlling weeds in a field containing the transgenic dicamba-tolerant plants of the invention and a method of decontaminating a material containing dicamba comprising applying an effective amount of a transgenic microorganism or dicamba-degrading enzyme(s) of the invention to the material. Finally, the invention provides a method of selecting transformed plants and plant cells based on dicamba tolerance and a method of selecting or screening transformed host cells, intact organisms and parts of organisms based on the fluorescence of 3,6-dichlorosalicylic acid produced as a result of dicamba degradation.

Claims

1. A recombinant DNA molecule, consisting essentially of a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence that differs from SEQ ID NO:

3 by one substitution.

2. A recombinant DNA molecule, consisting essentially of a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence that differs from SEQ ID NO:

3 by one addition.

3. A plant transformation vector, comprising a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence that differs from SEQ ID NO:

3 by one substitution.

8. A transgenic plant cell that has been transformed with the plant transformation vector of claim 3.

12. A transgenic plant or part of said transgenic plant comprising one or more cells transformed with the plant transformation vector of claim 3.

16. A transgenic seed from a transgenic plant according to claim 12.

4. A plant transformation vector, comprising a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence that differs from SEQ ID NO:

3 by one addition.

9. A transgenic plant cell that has been transformed with the plant transformation vector of claim 4.

13. A transgenic plant or part of said transgenic plant comprising one or more cells transformed with the plant transformation vector of claim 4.

17. A transgenic seed from a transgenic plant according to claim 13.

5. A plant transformation vector, comprising a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence that differs from SEQ ID NO:

3 by one substitution and by one addition.

10. A transgenic plant cell that has been transformed with the plant transformation vector of claim 5.

14. A transgenic plant or part of said transgenic plant comprising one or more cells transformed with the plant transformation vector of claim 5.

18. A transgenic seed from a transgenic plant according to claim 14.

6. A plant transformation vector, comprising a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence comprising SEQ ID NO:

3.

11. A transgenic plant cell that has been transformed with the plant transformation vector of claim 6.

15. A transgenic plant or part of said transgenic plant comprising one or more cells transformed with the plant transformation vector of claim 6.

19. A transgenic seed from a transgenic plant according to claim 15.

7. A plant transformation vector, comprising a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence comprises SEQ ID NO:

2.