Creatinine sensor calibration
First Claim
1. A method for calibrating a biosensor for the amperometric determination of creatinine in biological liquids, wherein the biosensor is integrated and operated in a device and has one electrode system each for measuring the concentrations of creatinine and creatine, with the electrode system for measuring the concentration of creatinine comprising creatininase, creatinase and sarcosine oxidase and the electrode system for measuring the concentration of creatine comprising creatinase and sarcosine oxidase, the method comprising:
- mixing an acidic creatinine solution with an alkaline buffer to form a creatinine calibration solution;
calibrating the electrode system for measuring the creatine concentration with a creatine calibration solution wherein creatinine and creatine are at a thermodynamic equilibrium; and
calibrating the electrode system for measuring creatinine concentration with the creatinine calibration solution, wherein the result of the electrode system for measuring the concentration of creatinine is corrected by the result of the electrode system for measuring the concentration of creatine.
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Abstract
In a method for calibrating a biosensor for the amperometric determination of creatinine in biological liquids, consisting of one electrode system each for measuring the concentrations of creatinine and creatine, with at least two calibration solutions being used, the electrode system for measuring the creatinine concentration is calibrated with a creatinine solution which, prior to the calibration measurement, was mixed in the form of an acidic solution with an alkaline buffer solution, and the electrode system for measuring the creatine concentration is calibrated with a solution in which creatinine and creatine are at a thermodynamic equilibrium.
13 Citations
20 Claims
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1. A method for calibrating a biosensor for the amperometric determination of creatinine in biological liquids, wherein the biosensor is integrated and operated in a device and has one electrode system each for measuring the concentrations of creatinine and creatine, with the electrode system for measuring the concentration of creatinine comprising creatininase, creatinase and sarcosine oxidase and the electrode system for measuring the concentration of creatine comprising creatinase and sarcosine oxidase, the method comprising:
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mixing an acidic creatinine solution with an alkaline buffer to form a creatinine calibration solution; calibrating the electrode system for measuring the creatine concentration with a creatine calibration solution wherein creatinine and creatine are at a thermodynamic equilibrium; and calibrating the electrode system for measuring creatinine concentration with the creatinine calibration solution, wherein the result of the electrode system for measuring the concentration of creatinine is corrected by the result of the electrode system for measuring the concentration of creatine. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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- 15. A method for calibrating a biosensor for the amperometric determination of creatinine in biological liquids, wherein the biosensor has one electrode system each for measuring the concentrations of creatinine and creatine, the method comprising providing a creatinine calibration solution and a creatine calibration solution for calibrating the electrodes, wherein the creatinine solution is prepared by mixing an acidic creatinine solution with an alkaline buffer solution, and the creatine calibration solution comprises a solution in which creatinine and creatine are at a thermodynamic equilibrium.
Specification