Electroluminescent illumination source for optical detection systems

• US 7,815,854 B2
• Filed: 12/22/2004
• Issued: 10/19/2010
• Est. Priority Date: 04/30/2004
• Status: Active Grant

• Current Assignee: Kimberly-Clark Worldwide Incorporated (Kimberly-Clark Corporation)
• Original Assignee: Kimberly-Clark Worldwide Incorporated (Kimberly-Clark Corporation)
• Inventors: Cohen, David Samuel
• Primary Examiner(s): Alexander; Lyle A
• Assistant Examiner(s): WHITE, DENNIS MICHAEL
• Application Number:
  US11/022,285
• Publication Number:
  US 20050244952A1
• Time in Patent Office: 2,127 Days
• Field of Search: 422/82.07, 422/56, 422/57, 422/82.05, 422/82.06, 435/6, 436/172, 436/169
• US Class Current: 422/423
• CPC Class Codes: G01N 21/01 Arrangements or apparatus for facilitating the optical investigation
• CPC Class Codes: G01N 21/66 electrically excited, e.g. electroluminescence
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence

Abstract

An optical detection system that utilizes an electroluminescent (EL) illumination source is provided. Unlike illumination sources used with some conventional optical detection systems, an EL device is relatively homogeneous and diffuse, and thus may provide uniform illumination to the test sample. In addition, the emitted light intensity of the EL device may be easily controlled by simply varying the voltage or the frequency of the applied current. The relatively flexibility of EL devices may also allow them to be readily incorporated into a chromatographic-based assay device for detecting the presence or absence of an analyte within a test sample.

Claims

1. An optical detection system for detecting the presence or quantity of an analyte within a test sample, said system comprising:

a lateral flow assay device that includes a porous membrane and a conjugate pad that is in fluid communication with said porous membrane, wherein said conjugate pad contains detection probes conjugated with an immunoreactive specific binding member, said detection probes being capable of producing a detection signal, wherein a receptive material is immobilized within a detection zone defined by said porous membrane and located downstream from said conjugate pad, said receptive material being configured to bind to said immunoreactive specific binding member or to the analyte;

an electroluminescent illumination source capable of providing electromagnetic radiation that causes said detection probes to produce said detection signal within said detection zone, wherein said electroluminescent illumination source is laminated to said porous membrane; and

a detector capable of registering said detection signal produced by said detection probes within said detection zone.

2. The optical detection system of claim 1, wherein an optically transparent adhesive is used to laminate said electroluminescent illumination source to said porous membrane, to a support for said porous membrane, or combinations thereof.

3. The optical detection system of claim 1, wherein said assay device is contained within a sample holder.

4. The optical detection system of claim 3, wherein said electroluminescent illumination source is also positioned within said sample holder.

5. The optical detection system of claim 4, further comprising a cartridge that contains leads for said electroluminescent illumination source, said cartridge defining a port that receives said sample holder.

6. The optical detection system of claim 1, wherein said electroluminescent illumination source is a dispersion or thin-film device.

7. The optical detection system of claim 1, wherein said electroluminescent illumination source contains a luminescent layer sandwiched between at least two electrodes.

8. The optical detection system of claim 7, wherein at least one of said electrodes contains carbon.

9. The optical detection system of claim 7, wherein at least one of said electrodes contains a metal selected from the group consisting of aluminum, gold, silver, copper, platinum, palladium, iridium, and alloys thereof.

10. The optical detection system of claim 7, wherein at least one of said electrodes contains an inorganic oxide.

11. The optical detection system of claim 10, wherein said inorganic oxide is selected from the group consisting of indium oxide, indium tin oxide, tin oxide, antimony tin oxide, and combinations thereof.

12. The optical detection system of claim 7, wherein said luminescent layer contains phosphor particles.

13. The optical detection system of claim 1, further comprising a dielectric layer positioned between said luminescent layer and at least one of said electrodes.

14. The optical detection system of claim 1, wherein said electroluminescent illumination source and said detector are positioned adjacent to said porous membrane.

15. The optical detection system of claim 1, wherein said electroluminescent illumination source and said detector are positioned on opposing sides of said porous membrane.

16. The optical detection system of claim 1, wherein said specific binding member is an antibody.

17. The optical detection system of claim 1, wherein said receptive material is configured to bind to the analyte.

18. The optical detection system of claim 1, wherein said receptive material is an antibody.

19. The optical detection system of claim 1, further comprising an absorbent pad that is configured to receive fluid that migrates laterally through said porous membrane.

20. The optical detection system of claim 1, wherein the membrane is formed from nitrocellulose.

21. An optical detection system for detecting the presence or quantity of an analyte within a test sample, said system comprising:

a lateral flow assay device that includes a porous membrane and a conjugate pad that is in fluid communication with said porous membrane, wherein said conjugate pad contains detection probes conjugated with an immunoreactive specific binding member, said detection probes being capable of producing a detection signal, wherein a receptive material is immobilized within a detection zone defined by said porous membrane and located downstream from said conjugate pad, said receptive material being configured to bind to said immunoreactive specific binding member or to the analyte;

an electroluminescent illumination source capable of providing electromagnetic radiation that causes said detection probes to produce said detection signal within said detection zone, wherein said electroluminescent illumination source is laminated to a support for said porous membrane; and

a detector capable of registering said detection signal produced by said detection probes within said detection zone.