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Microfluidic reaction apparatus for high throughput screening

  • US 7,815,868 B1
  • Filed: 02/28/2007
  • Issued: 10/19/2010
  • Est. Priority Date: 02/28/2006
  • Status: Expired due to Fees
First Claim
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1. A SBS-formatted microfluidic device comprising:

  • a plate having an upper surface and a lower surface, wherein the geometry of the plate defines an array of interrogation areas, wherein each interrogation area encompasses at least one reaction site; and

    an elastomeric microfluidic device comprising a network of microfluidic flow channels formed therein in fluid communication with an array of reaction sites configured to mix a plurality of fluids wherein the efficiency of mixing of the fluids is greater than 35%, the network of microfluidic flow channels comprising a set of reagent input channels and a set of sample input channels, the network of microfluidic flow channels further comprising, for each reaction sitea reaction chamber;

    a slug channel in communication with the reaction chamber, one of the reagent input channels, and one of the sample input channels;

    a first control valve positioned to open and close the slug channel at both its interface with the reaction chamber and its interface with the sample input channel; and

    a second control valve positioned to open and close the slug channel at its interface with the reagent input channel.

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