Castable diffusion membrane for enzyme-based sensor application

• US 7,816,112 B2
• Filed: 07/07/2004
• Issued: 10/19/2010
• Est. Priority Date: 07/11/2003
• Status: Active Grant

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: Desprez, Valerie
• Primary Examiner(s): Robinson; Hope A
• Application Number:
  US10/886,802
• Publication Number:
  US 20050009130A1
• Time in Patent Office: 2,295 Days
• Field of Search: None
• US Class Current: 435/195
• CPC Class Codes: C12M 1/34 Measuring or testing with condition measuring or sensing means, e.g. colony counters
• CPC Class Codes: C12N 11/093 Polyurethanes
• CPC Class Codes: C12Q 1/48 involving transferase
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 27/327 Biochemical electrodes , e.g. electrical or mechanical details for in vitro measurements
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose

Abstract

The invention relates to a diffusion membrane for an enzyme-based sensor, a sensor comprising the diffusion membrane as well as the use of the enzyme-based sensor for the detection and/or determination of a substance, in particular an enzyme substrate, e.g., glucose.

Claims

1. A diffusion membrane for an enzyme-based sensor application, wherein the membrane comprises:

at least one castable polymer material; and

porous pore-maker particles dispersed in the at least one castable polymer material, the porous pore-maker particles having a size of about 0.5 μ

m to about 100 μ

m.

2. The diffusion membrane of claim 1, wherein the porous pore-maker particles are present in an amount of about 0.5% to about 70% by weight.

3. The diffusion membrane of claim 1, wherein the porous pore-maker particles comprise diatomaceous earth.

4. The diffusion membrane of claim 1, wherein the thickness of the membrane is about 0.5 μ

m to about 1000 μ

m.

5. The diffusion membrane of claim 1, wherein the at least one castable polymer material is selected from the group consisting of polyurethane, polyacrylamide, polystyrene, polyvinyl esters, copolymers of butadiene and styrene, and mixtures thereof.

6. The diffusion membrane of claim 1, wherein the porous pore-marker particles comprise inorganic or organic particles selected from the group consisting of Kieselguhr, silica gel, cellulose, precipitated gypsum, kaolin, glass, silicon dioxide, and mixtures thereof.