Oral detection test for cannabinoid use
First Claim
Patent Images
1. A method for quantifying the amount of 11-nor-Δ
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9-THC carboxylic acid (“
THCA”
) in an oral fluid sample comprising the steps of;
providing an oral fluid sample;
ionizing any THCA present in the oral fluid sample under conditions suitable to produce one or more ions detectable by mass spectrometry, wherein said one or more ions detectable by tandem mass spectrometry comprise a THCA parent ion with a mass to charge ratio (m/z) of 620.5 or one or more THCA fragment ions selected from the group of ions with mass to charge ratio (m/z) of 492 and 383;
determining the amount of said one or more ions by tandem mass spectrometry; and
using the amount of said one or more ions to determine the amount of THCA in the sample.
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Abstract
A method for confirming the active intake of marijuana and its active component Δ9-tetrahydrocannabinol (“Δ9-THC”) by detecting the amount of 11-nor-Δ9-THC carboxylic acid (“THCA”) in oral fluid at the picogram per milliliter (pg/ml) level using chromatography/mass spectrometry/mass spectrometry (“GC/MS/MS”).
38 Citations
24 Claims
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1. A method for quantifying the amount of 11-nor-Δ
-
9-THC carboxylic acid (“
THCA”
) in an oral fluid sample comprising the steps of;providing an oral fluid sample; ionizing any THCA present in the oral fluid sample under conditions suitable to produce one or more ions detectable by mass spectrometry, wherein said one or more ions detectable by tandem mass spectrometry comprise a THCA parent ion with a mass to charge ratio (m/z) of 620.5 or one or more THCA fragment ions selected from the group of ions with mass to charge ratio (m/z) of 492 and 383; determining the amount of said one or more ions by tandem mass spectrometry; and using the amount of said one or more ions to determine the amount of THCA in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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9-THC carboxylic acid (“
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14. A method for quantifying the amount of 11-nor-Δ
-
9-THC carboxylic acid (“
THCA”
) in a sample comprising the steps of;providing a sample; purifying any THCA present in the sample by solid phase extraction; ionizing the purified THCA from the sample under conditions suitable to produce one or more THCA ions detectable by mass spectrometry; determining the amount of one or more THCA ions by tandem mass spectrometry; and using the amount of said one or more THCA ions to determine the amount of THCA in the sample; wherein said one or more THCA ions determined by tandem mass spectrometry comprise at least one THCA parent ion with a mass to charge ratio (m/z) of 620.5 and one or more THCA fragment ions selected from the group of ions with mass to charge ratio (m/z) of 492 and 383. - View Dependent Claims (15, 16, 17, 18, 19, 20)
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9-THC carboxylic acid (“
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21. A method for determining the amount of isotopically labeled 11-nor-Δ
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9-THC carboxylic acid (“
THCA”
) in a sample, said method comprising;providing a sample containing isotopically labeled THCA; ionizing said isotopically labeled THCA under conditions suitable to produce one or more ions detectable by mass spectrometry; determining the amount of one or more isotopically labeled THCA ions by tandem mass spectrometry; and using the amount of said one or more isotopically labeled THCA ions to determine the amount of said isotopically labeled THCA in the sample; wherein said one or more isotopically labeled THCA ions determined by tandem mass spectrometry comprise at least one isotopically labeled THCA parent ion having a mass to charge ratio (m/z) of 623.5, and one or more isotopically labeled THCA fragment ions. - View Dependent Claims (22, 23, 24)
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9-THC carboxylic acid (“
Specification