Method, device and apparatus for measuring the concentration of creatinine, and method, device and apparatus for measuring the amount of salt using the same

• US 7,816,145 B2
• Filed: 03/04/2010
• Issued: 10/19/2010
• Est. Priority Date: 05/16/2008
• Status: Active Grant

• Current Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Original Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Inventors: Nakaminami, Takahiro, Fukunaga, Atsushi, Kashiwada, Kouji
• Primary Examiner(s): Gakh; Yelena G
• Assistant Examiner(s): WEISZ, DAVID G
• Application Number:
  US12/717,493
• Publication Number:
  US 20100159606A1
• Time in Patent Office: 229 Days
• Field of Search: 436/98, 436/96, 436/91, 356/72
• US Class Current: 436/98
• CPC Class Codes: C12Q 1/34 involving hydrolase
• CPC Class Codes: G01N 21/80 Indicating pH value
• CPC Class Codes: G01N 33/5302 Apparatus specially adapted for immunological test procedures
• CPC Class Codes: G01N 33/70 involving creatine or creatinine
• CPC Class Codes: Y10T 436/14 Heterocyclic carbon compound [i.e. , O, S, N, Se, Te, as only ring hetero atom]
• CPC Class Codes: Y10T 436/145555 Hetero-N
• CPC Class Codes: Y10T 436/147777 Plural nitrogen in the same ring [e.g., barbituates, creatinine, etc.]

Abstract

A method for measuring a concentration of creatinine includes the steps of: (A) mixing a sample containing creatinine with a creatinine quantitative reagent containing a metal complex of at least one of hexacyanoferrate and hexacyanoruthenate in the absence of picric acid and any enzyme responsive to creatinine, to cause the creatinine to reduce the metal complex; (B) electrochemically or optically measuring the amount of the metal complex reduced in the step (A); and (C) determining the concentration of the creatinine contained in the sample from the amount of the reduced metal complex measured in the step (B).

Claims

1. A method for measuring a concentration of creatinine contained in urine or blood, comprising the steps of:

(A) mixing urine or blood containing creatinine with a creatinine quantitative reagent containing a metal complex of at least one of hexacyanoferrate and hexacyanoruthenate in the absence of picric acid and in the absence of any enzyme responsive to creatinine, to cause the creatinine to reduce the metal complex,

wherein, the urine or blood after the mixing in step (A) has a pH of not less than 2.5 and not more than 7;

(B) electrochemically measuring an amount of the metal complex reduced in step (A); and

(C) determining a concentration of the creatinine contained in the urine or blood from the amount of the reduced metal complex measured in step (B).

2. The method for measuring a concentration of creatinine in accordance with claim 1, wherein after the mixing in step (A), the urine or blood has a pH of 3 or more and 6 or less.

3. The method for measuring a concentration of creatinine in accordance with claim 1, wherein in step (A), the urine or blood is further mixed with a phosphate buffer.

4. The method for measuring a concentration of creatinine in accordance with claim 1, wherein in step (A), the urine or blood is further mixed with a phosphate buffer so that a pH of the sample is adjusted to 5 to 6.

5. The method for measuring a concentration of creatinine in accordance with claim 1, wherein in step (A), the urine or blood is further mixed with a cationic hydrophilic polymer.

6. The method for measuring a concentration of creatinine in accordance with claim 5, wherein the cationic hydrophilic polymer is cationic guar gum.

7. The method for measuring a concentration of creatinine in accordance with claim 1,

wherein step (B) comprises the steps of:

(D) bringing the urine or blood into contact with two or more electrodes and applying a voltage between the two electrodes; and

(E) detecting a current value or an amount of electric charge flowing between the two electrodes, and

in step (C), the concentration of the creatinine contained in the urine or blood is determined from the current value or the amount of electric charge detected in the step (E).