Compositions comprising a linked acceptor moiety

• US 7,816,501 B2
• Filed: 06/30/2003
• Issued: 10/19/2010
• Est. Priority Date: 03/24/1998
• Status: Active Grant

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Boston Probes, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Coull, James M., Gildea, Brian D., Hyldig-Nielsen, Jens J.
• Primary Examiner(s): Riley; Jezia
• Application Number:
  US10/610,338
• Publication Number:
  US 20090270602A1
• Time in Patent Office: 2,668 Days
• Field of Search: 536/23.1, 536/25.3, 536/26.6, 435/6
• US Class Current: 536/23.1
• CPC Class Codes: C07K 14/003 Peptide-nucleic acids (PNAs)
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: C12Q 1/6851 Quantitative amplification
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 2525/107 incorporating a peptide nucleic acid
• CPC Class Codes: C12Q 2561/113 Real time assay
• CPC Class Codes: C12Q 2565/101 Interaction between at least two labels

Abstract

This invention is directed to compositions comprising a linked acceptor moiety.

Claims

1. A non-nucleic acid polymer bearing a linked acceptor moiety but not a linked donor moiety, wherein the non-nucleic acid polymer comprises subunits having the formula:

2. The non-nucleic acid polymer of claim 1, wherein

each L is independently selected from the group consisting of J, adenine, cytosine, guanine, thymine, uridine, 5-methylcytosine, 2- aminopurine, 2-amino-6-chloropurine, 2,6-diaminopurine, hypoxanthine, pseudoisocytosine, 2-thiouracil, 2-thiothymidine, other naturally occurring nucleobase analogs, other non-naturally occurring nucleobases, substituted and unsubstituted aromatic moieties, biotin, and dabcyl.

3. The non-nucleic acid polymer of claim 2, wherein the non-nucleic acid polymer comprises a naturally occurring nucleobase attached to the aza nitrogen of N-[2-(aminoethyl)]glycine backbone through a methylene carbonyl linkage.

4. The non-nucleic acid polymer of claim 1, wherein the acceptor moiety is dabcyl.

5. The non-nucleic acid polymer of claim 1, wherein the acceptor moiety is a quencher moiety.

6. The non-nucleic acid polymer of claim 5, wherein the quencher moiety is dabcyl.