Compositions and methods of producing hybrid antigen binding molecules and uses thereof

• US 7,820,165 B2
• Filed: 11/21/2006
• Issued: 10/26/2010
• Est. Priority Date: 11/21/2005
• Status: Expired due to Fees

• Current Assignee: Merck Serono SA (Merck & Co., Inc.)
• Original Assignee: Merck Serono SA (Merck & Co., Inc.)
• Inventors: Campbell, Robert K., Schweickhardt, Rene Lynn, Kelton, Christie Ann, Yang, Meijia, He, Chaomei, Jiang, Xuliang, McKenna, Sean D., De Luca, Giampiero, Arkinstall, Stephen J.
• Primary Examiner(s): HUYNH, PHUONG N
• Application Number:
  US12/085,155
• Publication Number:
  US 20090130712A1
• Time in Patent Office: 1,435 Days
• Field of Search: None
• US Class Current: 424/134.1
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: C07K 16/22 against growth factors ; against growth regulators
• CPC Class Codes: C07K 16/2863 against receptors for growth factors, growth regulators
• CPC Class Codes: C07K 2317/56 variable (Fv) region, i.e. VH and/or VL
• CPC Class Codes: C07K 2318/10 Immunoglobulin or domain(s) thereof as scaffolds for inserted non-Ig peptide sequences, e.g. for vaccination purposes
• CPC Class Codes: C07K 2318/20 Antigen-binding scaffold molecules wherein the scaffold is not an immunoglobulin variable region or antibody mimetics
• CPC Class Codes: C07K 2319/00 Fusion polypeptide

Abstract

This disclosure relates to hybrid antigen binding molecules including at least two polypeptide chains, with at least one polypeptide chain comprises an antigen binding moiety linked to an amino acid sequence of a subunit of a heterodimeric proteinaceous hormone. Also disclosed are methods of making and using such hybrid antigen binding molecules for diagnosis and/or therapy.

Claims

1. An anti-EGFR fusion protein comprising the humanized EGFR-specific antigen binding moiety of SEQ ID NO.:

83 linked to a fusion partner.

2. The anti-EGFR fusion protein of claim 1 comprising the amino acid sequence of SEQ ID NO:

84.

3. The anti-EGFR fusion protein of claim 1 comprising the amino acid sequence of SEQ ID NO:

85.

4. An anti-EGFR binding protein comprising the amino acid sequence of SEQ ID NO:

83 or a EGFR-specific antigen binding moiety thereof.