Biolithographical deposition and materials and devices formed therefrom

• US 7,820,227 B2
• Filed: 02/20/2004
• Issued: 10/26/2010
• Est. Priority Date: 12/11/2003
• Status: Active Grant

• Current Assignee: University of Maryland Baltimore County (University of Maryland), University of Maryland, College Park
• Original Assignee: University of Maryland Baltimore County (University of Maryland), University of Maryland, College Park
• Inventors: Yi, Hyunmin, Ghodssi, Reza, Wu, Li-Qun, Payne, Gregory F., Bentley, William E., Fernandes, Rohan, Rubloff, Gary W.
• Primary Examiner(s): Barr; Michael
• Assistant Examiner(s): BOWMAN, ANDREW J
• Application Number:
  US10/581,905
• Publication Number:
  US 20070275246A1
• Time in Patent Office: 2,440 Days
• Field of Search: 427/2.1, 427/2.11, 427/2.12
• US Class Current: 427/2.1
• CPC Class Codes: A61L 31/10 Macromolecular materials
• CPC Class Codes: A61L 31/14 Materials characterised by their function or physical properties , e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
• CPC Class Codes: C08L 5/08 Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
• CPC Class Codes: G03F 7/40 Treatment after imagewise removal, e.g. baking
• CPC Class Codes: Y10T 428/31504 Composite [nonstructural laminate]
• CPC Class Codes: Y10T 428/31536 Including interfacial reaction product of adjacent layers
• CPC Class Codes: Y10T 428/31971 Of carbohydrate

Abstract

A method for biolithographical deposition of molecules is provided. According to an embodiment of the method, a reactive layer (e.g., a polysaccharide mass) having a surface region coated with a biologically compatible resist is provided. A portion of the biologically compatible resist is selectively removed to expose an exposed portion of the reactive layer. Molecules, such as biomolecules and/or cellular species, are then conjugated to the exposed portion of the reactive layer. Also provided are materials and devices related to the method.

Claims

1. A method for selective deposition of molecules, comprising the steps of:

(a) providing a reactive layer comprising a surface region coated with a resist that is biologically compatible with the reactive layer;

(b) selectively removing a portion of the biologically compatible resist from the surface region to expose an exposed portion of the reactive layer; and

(c) conjugating molecules with the exposed portion of the reactive layer;

wherein the reactive layer comprises chitosan.

2. The method of claim 1, wherein the reactive layer comprises a polysaccharide mass.

3. The method of claim 2, further comprising depositing a selectively insolubilizable polysaccharide on a substrate to form the polysaccharide mass.

4. The method of claim 3, wherein said depositing comprises providing an aqueous solution comprising the selectively insolubilizable polysaccharide and having an initial pH, contacting the aqueous solution with the substrate, and altering the pH of the aqueous solution to insolubilize and deposit the selectively insolubilized polysaccharide on the substrate as a film of the polysaccharide mass.

5. The method of claim 3, wherein said depositing comprises providing an aqueous solution comprising the selectively insolubilizable polysaccharide and having an initial pH, electrochemically depositing the selectively insolubilizable polysaccharide on an electrically conductive support of the substrate, and altering the pH of the aqueous solution to stabilize the selectively insolubilized polysaccharide on the electrically conductive support.

6. The method of claim 1, wherein the biologically compatible resist comprises a gelatin thermoresist.

7. The method of claim 1, wherein said selective removing comprises biolithography.

8. The method of claim 1, wherein the biologically compatible resist comprises a thermoresist, and wherein said selective removing comprises melting a portion of the thermoresist and removing the melted portion of the thermoresist from the reactive layer.

9. The method of claim 8, where said melting is performed with a heated stamp applied to an exposed face of the thermoresist.

10. The method of claim 8, wherein said melting is performed with heating means incorporated in the solid support beneath the thermoresist.

11. The method of claim 1, wherein said selective removing comprises enzymatically removing a portion of the biologically compatible resist.

12. The method of claim 1, further comprising modifying the reactive layer to improve conjugatability with reactive groups of the molecules.

13. The method of claim 1, further comprising modifying the molecules to improve conjugatability with reactive groups of the reactive layer.

14. The method of claim 1, wherein the molecules comprise biomolecules.

15. The method of claim 14, wherein the molecules comprise one, two, three or more protein species.

16. The method of claim 14, wherein the molecules comprise one, two, three or more enzyme species.

19. The method of claim 1, wherein the molecules comprise one, two, three or more nucleic acid molecule species.

20. The method of claim 1, wherein the exposed portion and the molecules comprise a first exposed portion and a first molecular species, respectively, and wherein the method further comprises the steps of:

(d) coating the biologically compatible resist on the first molecular species conjugated to the first exposed portion of the reactive layer;

(e) selectively removing a second portion of the biologically compatible resist to expose a second exposed portion of the reactive layer; and

(f) conjugating a second molecular species with the second exposed portion of the reactive layer.

21. The method of claim 20, wherein the first and second molecular species are conjugated with the reactive layer sequentially.

17. A method for selective deposition of molecules, comprising the steps of:

(a) providing a reactive layer comprising a surface region coated with a resist that is biologically compatible with the reactive layer;

(b) selectively removing a portion of the biologically compatible resist from the surface region to expose an exposed portion of the reactive layer; and

(c) conjugating molecules with the exposed portion of the reactive layer;

wherein the molecules comprise one, two, three or more antibody species.

18. A method for selective deposition of molecules, comprising the steps of:

(a) providing a reactive layer comprising a surface region coated with a resist that is biologically compatible with the reactive layer;

(b) selectively removing a portion of the biologically compatible resist from the surface region to expose an exposed portion of the reactive layer; and

(c) conjugating molecules with the exposed portion of the reactive layer;

wherein the molecules comprise one, two, three or more receptor molecule species.

22. A method for selective deposition of molecules, comprising the steps of:

(a) providing a reactive layer comprising a surface region coated with a resist that is biologically compatible with the reactive layer;

(b) selectively removing a portion of the biologically compatible resist from the surface region to expose an exposed portion of the reactive layer; and

(c) conjugating molecules with the exposed portion of the reactive layer;

wherein the reactive layer comprises chitosan and wherein the molecules comprise one, two, three or more antibody species.

23. A method for selective deposition of molecules, comprising the steps of:

(a) providing a reactive layer comprising a surface region coated with a resist that is biologically compatible with the reactive layer;

(b) selectively removing a portion of the biologically compatible resist from the surface region to expose an exposed portion of the reactive layer; and

(c) conjugating molecules with the exposed portion of the reactive layer;

wherein the reactive layer comprises chitosan and wherein the molecules comprise one, two, three or more receptor molecule species.