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Incrementally truncated nucleic acids and methods of making same

  • US 7,820,413 B2
  • Filed: 02/19/2008
  • Issued: 10/26/2010
  • Est. Priority Date: 11/15/2000
  • Status: Expired due to Fees
First Claim
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1. A method of making a plurality of incrementally truncated hybrid nucleic acids comprising the steps of:

  • a) serially removing nucleotides from one or both termini of a first and a second parent nucleic acid to form truncated first and second parent nucleic acids whose length decreases incrementally over time;

    b) stopping said serial nucleotide removal at a plurality of different times to form a plurality of incrementally truncated first and second parent nucleic acids;

    c) linking separate incrementally truncated first parent nucleic acids to separate incrementally truncated second parent nucleic acids to form a plurality of incrementally truncated hybrid nucleic acids.

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