Compositions including ammonia oxidizing bacteria to increase production of nitric oxide and nitric oxide precursors and methods of using same

• US 7,820,420 B2
• Filed: 08/10/2001
• Issued: 10/26/2010
• Est. Priority Date: 08/11/2000
• Status: Expired due to Fees

• Current Assignee: AOBiome LLC (AOBiome Therapeutics, Inc.)
• Original Assignee: David R. Whitlock
• Inventors: Whitlock, David R.
• Primary Examiner(s): Weber; Jon P
• Assistant Examiner(s): Srivastava; Kailash C
• Application Number:
  US10/332,933
• Publication Number:
  US 20040014188A1
• Time in Patent Office: 3,364 Days
• Field of Search: 435/6, 435/4, 435/170, 438/149
• US Class Current: 435/170
• CPC Class Codes: A23L 13/72 using additives, e.g. by injection of solutions
• CPC Class Codes: A61K 33/00 Medicinal preparations containing inorganic active ingredients
• CPC Class Codes: A61K 35/741 Probiotics probiotic yeast, e.g. saccharomyces A61K36/06
• CPC Class Codes: A61K 8/0208 Tissues; Wipes; Patches
• CPC Class Codes: A61K 8/19 containing inorganic ingredients
• CPC Class Codes: A61K 8/365 Hydroxycarboxylic acids; Ketocarboxylic acids
• CPC Class Codes: A61K 8/42 Amides
• CPC Class Codes: A61K 8/99 from microorganisms other than algae or fungi, e.g. protozoa or bacteria
• CPC Class Codes: A61K 9/70 Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug hollow drug-filled fibres A61K9/0092
• CPC Class Codes: A61L 15/36 containing microorganisms
• CPC Class Codes: A61P 15/08 for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
• CPC Class Codes: A61P 15/10 for impotence
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: A61Q 15/00 Anti-perspirants or body deodorants deodorisation of air A61L9/00
• CPC Class Codes: C12P 3/00 Preparation of elements or inorganic compounds except carbon dioxide recovery of carbon dioxides as by-products C12F3/02
• CPC Class Codes: D06M 16/00 Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
• CPC Class Codes: D06M 16/003 with enzymes or microorganisms

Abstract

A method of enhancing health through the generation in close proximity of a surface of a subject, nitric oxide and nitric oxide precursors using bacteria adapted to oxidize ammonia and urea derived from perspiration is described. Local and systemic effects are described including reduction in vascular disease, enhancement of sexual function, improved skin health, and reduced transmission of sexually transmitted diseases.

Claims

1. A method of supplying nitric oxide to a subject comprising:

applying ammonia oxidizing bacteria to a surface of the subject in an effective amount to cause the bacteria to metabolize a component selected from the group consisting of ammonia, ammonium salts and urea on the surface into any of the nitric oxide, a nitric oxide precursor, or combinations thereof.

2. The method of claim 1, wherein the act of applying the bacteria comprises applying the bacteria in a carrier.

3. The method of claim 1, wherein the act of applying the bacteria comprises applying a bacteria selected from the group consisting of any of Nitrosomonas, Nitrosococcus, Nitrosospira, Nitrosocystis, Nitrosolobus, Nitrosovibrio, and combinations thereof.

4. The method of claim 1, wherein the act of applying the bacteria to a surface comprises applying the bacteria to skin, hair, or a combination thereof.

5. The method of claim 1, wherein the act of applying the bacteria comprises applying a substantially homogeneous autotrophic culture of bacteria.

6. The method of claim 1, wherein the act of applying the bacteria comprises:

applying the bacteria to an article;

contacting the article with the surface of the subject; and

transferring at least a portion of the bacteria from the article to the surface of the subject.

7. The method of claim 1, wherein the act of applying the bacteria comprises applying the bacteria mixed with an acid.

8. The method of claim 1, wherein the act of applying further comprises metabolizing any of urine, feces, blood, wound secretions, menstrual secretions, vaginal secretions, topically applied mixtures, and combinations thereof.

9. The method of claim 1, further comprising the act of applying a compound selected from the group consisting of perspiration, urea, nitrite, lactic acid, nitrate, salt, iron salts, ammonium salts, and combinations thereof, to the surface of the subject.

10. The method of claim 1, wherein the act of applying the bacteria comprises applying the bacteria to a subject that is a non-human vertebrate.

11. The method of claim 1, further comprising:

applying at least one of urea or mineral salts to the surface of the subject in an effective amount to stimulate growth of the bacteria.

12. The method of claim 4, wherein the act of applying the bacteria to a surface of a subject includes applying the bacteria to the skin, hair, or a combination thereof, of a human.

13. The method of claim 4, wherein the act of applying the bacteria to the surface of the subject comprises applying the bacteria to the surface of the subject having a disorder selected from any of a vascular disease, impotence, infertility, or combinations thereof.

14. The method of claim 6, wherein the act of contacting the article with the surface of the subject further comprises contacting a fluid excretion from the surface of the subject with the bacteria.

15. The method of claim 6, wherein the act of applying the bacteria to an article comprises applying the bacteria to an article of clothing.

16. The method of claim 6, wherein the act of applying the bacteria mixed with an acid comprises applying the bacteria mixed with lactic acid.

17. The method of claim 9, wherein the acts of applying the compound and the bacteria include applying compound and the bacteria in a mixture.

18. The method of claim 10, wherein the act of applying the bacteria to the surface of the non-human vertebrate comprises:

applying the bacteria to any of skin, feather, hair, fur, scale or combinations thereof.

19. The method of claim 12, wherein the act of applying the bacteria comprises applying the bacteria to an underarm surface.

20. The method of claim 15, wherein the act of applying the bacteria to the article of clothing comprises the acts of:

wearing the article and impregnating the article with the bacteria.

21. The method of claim 15, wherein the act of applying the bacteria to the article of clothing comprises the acts of:

wearing the article and coating the article with the bacteria.

22. The method of claim 15, wherein act of applying the bacteria comprises applying the bacteria to a diaper.

23. The method of claim 15, wherein the act of applying the bacteria comprises applying the bacteria to an article of clothing selected from the group consisting of shoe, sneaker, belt, hat, undergarment, pajama, athletic garment, sock, shoe insert, bandage, face mask, scarf, tampon, and condom.

24. The method of claim 18, wherein the act of applying the bacteria comprises applying the bacteria to any of bedding, litter, an article of clothing, or a habitat.

25. The method of claim 20, wherein the act of impregnating the article includes contacting the article with a culture medium containing the bacteria.

26. The method of claim 25, wherein the act of contacting the article with a culture medium containing the bacteria comprises applying an animal manure.

27. The method of claim 25, wherein the act of contacting the article with a culture medium containing the bacteria comprises contacting the article with a substantially pure culture of bacteria.

28. The method of claim 1, wherein the act of applying the bacteria comprises:

applying the ammonia oxidizing bacteria to the surface of the subject in an amount effective to metabolize a normal bodily secretion on the surface into any of nitric oxide, nitric oxide precursors, or combinations thereof.

29. The method of claim 28, wherein the act of applying bacteria comprises applying the bacteria to the skin between intervals of bathing.

30. The method of claim 28, wherein the act of metabolizing a normal constituent of skin secretion comprises metabolizing a component of perspiration.

31. The method of claim 29, wherein the act of applying the bacteria between intervals of bathing includes applying the bacteria at an interval of at least about one day.

32. The method of claim 30, wherein the act of metabolizing comprises metabolizing a component of perspiration selected from the group consisting of urea, nitrite, lactic acid, nitrate, salt, iron salts, ammonium salts, and combinations thereof.

33. The method of claim 29, wherein the act of applying the bacteria between intervals of bathing comprises applying the bacteria at an interval that is greater than or equal to about one week.

34. The method of claim 29, wherein the act of applying the bacteria is applied to a subject who lacks bathing facilities, is immobile, or has a bandage.

35. The method of claim 28, wherein the act of applying the bacteria comprises locally applying the bacteria to genitalia.

36. The method of claim 35, wherein the act of applying the bacteria includes locally applying the bacteria to a penis.

37. The method of claim 35, where in the act of applying the bacteria comprises locally applying the bacteria to a vagina.

38. The method of claim 1, further comprising applying a component selected from the group consisting of urea, ammonium salts, sodium, potassium, magnesium, calcium, phosphate, chloride, sulfate, trace mineral salts, iron, copper, zinc, cobalt, manganese, molybdenum, buffers, and combinations thereof to the surface of the subject in an effective amount to stimulate growth of the bacteria.

39. A topical preparation for application to a surface of a subject comprising ammonia oxidizing bacteria that metabolizes perspiration when present, into any of nitric oxide, nitric oxide precursors, or combinations thereof.

40. The preparation of claim 39, wherein the preparation is any of a cosmetic composition, a body deodorant, or an athletic preparation.

41. The preparation of claim 39, wherein the bacteria is selected from the group consisting of Nitrosomonas, Nitrosococcus, Nitrosospira, Nitrosocystis, Nitrosolobus, Nitrosovibrio, and combinations thereof.

42. The preparation of claim 39, wherein the component of perspiration is selected from the group consisting of urea, nitrite, lactic acid, nitrate, salt, iron salts, ammonium salts, and combinations thereof.

43. The preparation of claim 40, further comprising at least one component selected from the group consisting of water, mineral oil, coloring agent, perfume, aloe, glycerin, sodium chloride, pH buffers, UV absorbing agents, silicone oil, natural oil, vitamin E, herbal concentrates, Lactic acid, citric acid, talc, clay, calcium carbonate, magnesium carbonate, zinc oxide, starch, urea, nitrite, nitrate, iron salts, ammonium salts, and combinations thereof.

44. The preparation of claim 40, wherein the preparation is any of powder, cream, stick, aerosol, or salve.

45. The preparation of claim 39, wherein the surface of the subject is of a human being.

46. The preparation of claim 39, further comprising a component selected from the group consisting of water, mineral oil, coloring agent, perfume, aloe, glycerin, sodium chloride, pH buffers, UV absorbing agents, silicone oil, natural oils, vitamin E, herbal concentrates, lactic acid, citric acid, talc, clay, calcium carbonate, magnesium carbonate, zinc oxide, starch, urea, nitrite, lactic acid, nitrate, iron salts, ammonium salts, and combinations thereof.

47. The preparation of claim 39, further comprising:

at least one compound selected from the group consisting of urea, ammonium salts, sodium, potassium, magnesium, calcium, phosphate, chloride, sulfate, trace mineral salts, iron, copper, zinc, cobalt, manganese, molybdenum, buffers, and combinations thereof.