Microfluidic device and methods of using same
First Claim
1. A method comprising(a) introducing a sample solution comprising a sample and reagents into a microfluidic device, wherein the device comprises:
- (i) an unbranched flow channel in fluid communication with two fluid inlets, wherein a solution can be introduced into said flow channel or withdrawn from said flow channel through said inlets; and
(ii) a plurality of valves that, when closed, partition said flow channel into isolated reaction sites, said reaction sites being present at a density of at least 50 sites/cm2;
(b) closing said valves, thereby partitioning the flow channel into isolated reaction sites containing portions of said sample solution;
(c) exposing the reaction sites to conditions under which a reaction product is formed in at least one of the reaction sites; and
,(d) opening said valves and displacing at least a portion of the sample solution out of the flow channel.
1 Assignment
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Accused Products
Abstract
A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
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Citations
2 Claims
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1. A method comprising
(a) introducing a sample solution comprising a sample and reagents into a microfluidic device, wherein the device comprises: -
(i) an unbranched flow channel in fluid communication with two fluid inlets, wherein a solution can be introduced into said flow channel or withdrawn from said flow channel through said inlets; and (ii) a plurality of valves that, when closed, partition said flow channel into isolated reaction sites, said reaction sites being present at a density of at least 50 sites/cm2; (b) closing said valves, thereby partitioning the flow channel into isolated reaction sites containing portions of said sample solution; (c) exposing the reaction sites to conditions under which a reaction product is formed in at least one of the reaction sites; and
,(d) opening said valves and displacing at least a portion of the sample solution out of the flow channel. - View Dependent Claims (2)
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Specification