Systems and methods for high-throughput radiation biodosimetry
First Claim
1. A high-throughput method of analyzing a plurality of biological samples, comprising:
- (a) marking an identifier onto each of a plurality of capillary vessels;
(b) collecting a biological sample in each of the plurality of capillary vessels;
(c) transporting a receptacle to a centrifuge using a first robotic device, said receptacle containing the plurality of capillary vessels;
(d) extracting a predetermined component from each biological sample;
(e) image scanning the plurality of biological samples, said image scanning comprising detecting an image of each sample with an optical device;
(f) focusing the image with the optical device; and
(g) determining a radiation exposure of an organism from which the sample originated by analyzing the focused image.
2 Assignments
0 Petitions
Accused Products
Abstract
Systems and methods for high-throughput radiation biodosimetry are disclosed herein. In some embodiments, a high-throughput methods of analyzing a population for radiation exposure can include, in various possible sequences: marking a first capillary designed to retain a first sample from the population with a first identifier; transporting a plurality of samples to a biodosimetry system; inputting the samples into the biodosimetry system; centrifuging the plurality of samples including the first sample wherein each sample can be retained in a capillary and the first sample can be retained in the first capillary; transferring the plurality of capillaries including the first capillary from the centrifuge to a cutting device using a robotic device; cutting the first capillary; reading the first identifier; transferring at least one portion of the first sample from the first capillary to a well in an array, wherein the array can include one or more filters in a multi-well plate; correlating the first identifier to a location of the array that includes the at least one portion of the first sample; one or more cycles of biological processing, which can include addition of a reagent and/or incubation of a selected temperature such as, for example, 37° C., 4° C., room temperature, and the like; sealing the array; positioning the array adjacent to an imaging element; focusing the imaging element; capturing an image of the first sample in the array; and analyzing the image to determine whether the first sample indicates a level of radiation exposure exceeding a predetermined threshold.
-
Citations
22 Claims
-
1. A high-throughput method of analyzing a plurality of biological samples, comprising:
-
(a) marking an identifier onto each of a plurality of capillary vessels; (b) collecting a biological sample in each of the plurality of capillary vessels; (c) transporting a receptacle to a centrifuge using a first robotic device, said receptacle containing the plurality of capillary vessels; (d) extracting a predetermined component from each biological sample; (e) image scanning the plurality of biological samples, said image scanning comprising detecting an image of each sample with an optical device; (f) focusing the image with the optical device; and (g) determining a radiation exposure of an organism from which the sample originated by analyzing the focused image. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 18)
-
-
2. An apparatus for high-throughput analysis of a plurality of biological samples, comprising:
-
(a) means for marking an identifier onto each of a plurality of capillary vessels; (b) a plurality of capillary vessels, each containing a single one of the plurality of biological samples; (c) a robotic device for transporting a receptacle to a centrifuge, said receptacle containing the plurality of capillary vessels; (d) an extraction apparatus for extracting a predetermined component from each biological sample; (e) an image scanning apparatus for detecting an image of the extracted component; (f) a focusing apparatus for focusing the image with an optical device; and (g) a processor for determining a radiation exposure of an organism from which the sample originated by analyzing the focused image. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 19)
-
-
3. A system for high-throughput analysis of a plurality of biological samples, comprising:
-
(a) means for marking an identifier onto each of a plurality of capillary vessels, each capillary vessel containing a single one of the plurality of biological samples; (b) means for transporting a receptacle to a centrifuge, said receptacle containing the plurality of capillary vessels; (c) means for extracting a predetermined element from each biological sample, each said extracted element being correlated to a respective source of the biological sample; (d) means for detecting an image of each biological sample; (e) means for focusing the image; and (g) means for analyzing the focused image to determine a radiation exposure of an organism, said organism being the source of the biological sample. - View Dependent Claims (20, 21, 22)
-
Specification