Liquid portioning method and device

• US 7,823,535 B2
• Filed: 09/29/2003
• Issued: 11/02/2010
• Est. Priority Date: 09/27/2002
• Status: Expired due to Fees

• Current Assignee: Shimadzu Corporation
• Original Assignee: Shimadzu Corporation
• Inventors: Yamaguchi, Ryo, Furuta, Masaru, Akinaga, Nobuyuki, Sugiyama, Kiyohiro, Hanafusa, Nobuhiro, Miyamoto, Keisuke, Konoshita, Ryuh
• Primary Examiner(s): KOCH, GEORGE R
• Application Number:
  US10/529,005
• Publication Number:
  US 20060144331A1
• Time in Patent Office: 2,591 Days
• Field of Search: 118/665, 118/676, 118/677, 118/679, 118/688, 118/712, 118/713
• US Class Current: 118/665
• CPC Class Codes: B01L 2200/143 Quality control, feedback systems
• CPC Class Codes: B01L 2200/148 Specific details about calibrations
• CPC Class Codes: B01L 2300/0832 cylindrical, tube shaped
• CPC Class Codes: B01L 2400/0439 ultrasonic vibrations, vibrating piezo elements
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: B01L 3/0275 Interchangeable or disposable dispensing tips
• CPC Class Codes: G01N 2035/1041 Ink-jet like dispensers
• CPC Class Codes: G01N 35/1011 Control of the position or alignment of the transfer device
• CPC Class Codes: G01N 35/1016 Control of the volume dispensed or introduced

Abstract

Methods and apparatuses for dispensing samples or reagents onto a target object are provided. A dispensing position designating section (62) designates the dispensing position on the target object (50), such as a membrane, based on an image of the target object (50) displayed on a monitoring section (60). A dispensing control section (64) positions the target object and the dispensing device relative to each other so that the designated dispensing position on the target object is placed beneath the dispensing device (10) and controls the dispensing operation of the dispensing device (10). A dispensing position information creating section (68) creates dispensing position information about the dispensing position on the target object (50) which is designated by the dispensing position designating section (62) and to which the dispensing operation has been performed and outputs it to the outside.

Claims

1. A liquid dispensing apparatus comprising:

a dispensing mechanism including a plurality of dispensing devices for dripping a sample or a reagent;

an image capturing device for capturing the downward area image;

a movable table capable of supporting, on its upper surface, a target object onto which the sample or reagent is to be dispensed and being moved on a horizontal plane surface for positioning the target object at least at a dispensing position beneath the dispensing device and at an image capturing position beneath the image capturing device;

a monitoring section for displaying the image captured by the image capturing device;

a dispensing position designating section for designating the dispensing position on the target object based on the image of the target object displayed on the monitoring section;

a dispensing control section for positioning the target object and the dispensing device relative to each other so that the dispensing position on the target object designated by the dispensing position designating section is placed beneath the dispensing device, which performs the dispensing operation, of the dispensing mechanism and for controlling the dispensing operation of the dispensing mechanism;

the image capturing device for photographing the tip end of the dispensing device; and

a moving mechanism for moving the image capturing device in association with the switching of the dispensing device performing the dispensing operation so that the image capturing device is capable of photographing the tip end of the dispensing device performing the dispensing operation.

2. A liquid dispensing apparatus according to claim 1, further comprising a dispensing position information creating section for creating dispensing position information about the dispensing position on the target object which is designated by the dispensing position designating section and for which the dispensing operation has been performed.

3. A liquid dispensing apparatus according to claim 2, wherein the dispensing position information creating section is capable of outputting the created dispensing position information to the outside.

4. A liquid dispensing apparatus according to claim 2, wherein the dispensing position designating section designates the dispensing position on the image of the target object displayed on the monitoring section.

5. A liquid dispensing apparatus according to claim 1, wherein the dispensing position information creating section is capable of outputting the created dispensing position information to the outside.

6. A liquid dispensing apparatus according to claim 5, wherein the dispensing position designating section designates the dispensing position on the image of the target object displayed on the monitoring section.

7. A liquid dispensing apparatus according to claim 1, wherein the dispensing position designating section designates the dispensing position on the image of the target object displayed on the monitoring section.

8. A dispensing apparatus comprising:

a dispensing mechanism including a detachable dispensing device for dripping a sample or reagent;

an image capturing device for capturing downward area image;

a movable table capable of supporting, on its upper surface, a target object onto which the sample or reagent is to be dispensed and being moved on a horizontal plane surface for positioning the target object at least at a dispensing position beneath the dispensing device and at an image capturing position beneath the image capturing device; and

a calibrating section which, after liquid is dispensed onto a predetermined position on the movable table by the dispensing mechanism, detects the dispensing position based on the image captured by the image capturing device and calibrates the dispensing position based on base points serving as references on the movable table, the base points being captured concurrently with the image,

wherein the dispensing device has a discharging port at its lower end, and

wherein the dispensing mechanism further comprising;

a dispensing device retaining portion fixed to a main body of the liquid dispensing apparatus, the retaining portion arranging the dispensing device with the discharging port facing downwardly;

dispensation liquid container arranged on the dispensing device retained by the retaining portion to communicate to the dispensing device;

an air-introducing head arranged on the dispensation liquid container for communicating to the dispensation liquid container to adjust an inner pressure of the dispensation liquid container; and

an arm mechanism including an arm and a lock, the arm being supported rotatably at its base end on the main body to mount the air-introducing head detachably, the lock being capable of fixing the arm to the main body so that the air-introducing head is mounted on the dispensing device retained by the retaining portion through the dispensation liquid container and capable of releasing the fixing.

9. A liquid dispensing apparatus according to claim 8, wherein the dispensing mechanism includes a plurality of dispensing devices and the calibrating section performs the calibration for the respective dispensing devices.

10. A dispensing apparatus comprising:

a dispensing unit including a piezo chip having a downward opening portion at its discharging portion, the piezo chip being configured to discharge liquid from the discharging portion when liquid charged in a space communicated to the discharging portion is pushed by a driving section including a piezo device,

a pressure control mechanism for adjusting the pressure of the liquid charged in the space;

an image capturing device for capturing an image of the discharging portion;

a storing device for storing the image captured by the image capturing device; and

a control device which compares the image of the discharging portion captured before charging liquid in the space and stored in the storing device with images captured after charging liquid in the space and controls the pressure control mechanism so that, when liquid exits from the discharging portion, the liquid is retracted until the differences between the images and the image captured before charging liquid is cancelled.

11. A dispensing apparatus according to claim 10, wherein the image capturing device is installed so that it captures the image of the discharging portion along a horizontal direction.