Glucose level measuring method and glucose sensor utilizing glucose dehydrogenase

• US 7,824,881 B2
• Filed: 01/10/2008
• Issued: 11/02/2010
• Est. Priority Date: 06/17/2002
• Status: Expired due to Term

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Tsujimoto, Tomomichi, Yamaoka, Hideaki, Hoshijima, Mitsuhiro
• Primary Examiner(s): Weber; Jon P
• Assistant Examiner(s): Shen; Bin
• Application Number:
  US12/008,358
• Publication Number:
  US 20080131919A1
• Time in Patent Office: 1,027 Days
• Field of Search: None
• US Class Current: 435/26
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: C12Q 1/32 involving dehydrogenase
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

The present invention relates to a technique for measuring a glucose level by utilizing a reaction system containing an enzyme and an electron carrier. In accordance with the glucose level measuring method of the present invention, glucose dehydrogenase with cytochrome C attached thereto or glucose dehydrogenase derived from a microorganism belonging to a burkholderia genus is used as the enzyme, and a Ru compound is used as the electron carrier. The present invention further provides a glucose sensor in which glucose dehydrogenase with cytochrome C attached thereto or glucose dehydrogenase derived from derived from a microorganism belonging to a burkholderia genus is used as the enzyme, and a Ru compound is used as the electron carrier.

Claims

1. A glucose level measuring method using glucose dehydrogenase for measuring glucose level by utilizing a reaction system containing an enzyme and an electron carrier,

wherein the enzyme is glucose dehydrogenase derived from a microorganism belonging to burkholderia genus;

and

wherein the electron carrier is a Ru compound represented by a chemical formula as follows;

[Ru(NH₃)₅X]ⁿ⁺

where X represents NH3, halogen ion, CN, pyridine, nicotinamide or H₂O, and n+ represents a valency of the Ru complex, which is determined by X;

the method comprising;

causing the glucose dehydrogenase to oxidize the glucose while reducing the Ru compound, for producing a response; and

computing the glucose level based on the response.

2. The glucose level measuring method according to claim 1, wherein the glucose level measurement comprises subjecting the reaction system to a voltage, detecting response and computing the glucose level based on a detected level of the response.

3. The glucose level measuring method according to claim 1, wherein the glucose dehydrogenase includes an α

subunit having a glucose dehydrogenase activity and a molecular weight of 60 kDa in SDS-polyacrylamide gel electrophoresis under a reduced condition.

4. The glucose level measuring method according to claim 1, wherein the glucose dehydrogenase includes a γ

subunit having a molecular weight of 14 kDa in SDS-polyacrylamide gel electrophoresis under a reduced condition.