Compositions of aminoacyl-tRNA synthetase and uses thereof
First Claim
Patent Images
1. An isolated cell comprising a translation system where the translation system comprises a DNA sequence encoding the aminoacyl tRNA synthetase (RS) of SEQ ID NO:
- 5.
1 Assignment
0 Petitions
Accused Products
Abstract
Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA'"'"'s, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA'"'"'s/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.
-
Citations
19 Claims
- 1. An isolated cell comprising a translation system where the translation system comprises a DNA sequence encoding the aminoacyl tRNA synthetase (RS) of SEQ ID NO:
- 12. A vector comprising a DNA sequence encoding the RS of SEQ ID NO:
-
16. A method of producing a polypeptide in a cell with a selected amino acid at a specified position, the method comprising:
growing, in an appropriate medium, the cell, where the cell comprises a nucleic acid that comprises at least one selector codon and encodes a polypeptide; and
,providing the selected amino acid; wherein the cell further comprises; a DNA sequence that encodes an orthogonal RS (O-RS) that functions in the cell wherein said orthogonal RS has the amino acid sequence of SEQ ID NO;
5, and,an orthogonal tRNA (O-tRNA), that recognizes the selector codon wherein said O-RS aminoacylates the O-tRNA with the selected amino acid. - View Dependent Claims (17, 18)
-
19. An isolated polynucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 5, and the complementary polynucleotide sequence thereof.
Specification