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Identification and quantification of a plurality of biological (micro)organisms or their components

  • US 7,829,313 B2
  • Filed: 05/24/2006
  • Issued: 11/09/2010
  • Est. Priority Date: 03/24/2000
  • Status: Expired due to Fees
First Claim
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1. A diagnostic and/or quantification kit of a biological organism or part of an organism comprising:

  • (a) specific primers for the amplification of a nucleotide sequence;

    (b) a fluorescent label for labelling the amplified sequence;

    (c) an insoluble solid support surface upon which single-stranded unlabeled capture molecules are covalently bound, said capture molecules being disposed upon the surface of the solid support according to an array, wherein said capture molecules comprise a spacer portion comprising a nucleotide sequence of at least 40 bases in length and a capture portion of between 10 and 60 bases which is able to specifically bind to a nucleotide sequence of said organism or part of it; and

    (d) a closed reaction chamber for performing a genetic amplification together with the identification and/or quantification of amplified nucleotide sequences from said organism or part of it.

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