Assay devices that utilize hollow particles
First Claim
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1. An assay device for detecting an analyte in a test sample, the assay device comprising:
- a porous membrane that defines a detection zone, wherein a biological receptive material is immobilized within the detection zone; and
detection probes that contain latex particles having an average size of from about 0.1 nanometer to about 100 microns, wherein the latex particles individually define a polymeric shell about a hollow interior constituting at least about 20% of the spatial volume occupied by the particle, wherein the polymeric shell of the latex particles comprises an organic polymer covalently conjugated with a specific binding member for the analyte, wherein the organic polymer is covalently conjugated with the specific binding member via a carboxylic functional group on the organic polymer, and wherein any remaining activated carboxylic functional groups on the organic polymer of the polymeric shell are blocked;
wherein the detection probes are capable of generating a detection signal when present within the detection zone.
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Abstract
Hollow particles for use in various types of assay devices are provided. Due to their hollow or voided structure, the particles may exhibit a variety of beneficial properties. For instance, hollow particles are generally lightweight, and thus, relatively inexpensive in comparison to other types of particles. Hollow particles may also form a stable system without requiring refrigeration or rotation. In addition, hollow particles may possess enhanced light diffraction capabilities, which may be particularly beneficial in certain types of assay devices, e.g., diffraction-based assay devices.
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14 Claims
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1. An assay device for detecting an analyte in a test sample, the assay device comprising:
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a porous membrane that defines a detection zone, wherein a biological receptive material is immobilized within the detection zone; and detection probes that contain latex particles having an average size of from about 0.1 nanometer to about 100 microns, wherein the latex particles individually define a polymeric shell about a hollow interior constituting at least about 20% of the spatial volume occupied by the particle, wherein the polymeric shell of the latex particles comprises an organic polymer covalently conjugated with a specific binding member for the analyte, wherein the organic polymer is covalently conjugated with the specific binding member via a carboxylic functional group on the organic polymer, and wherein any remaining activated carboxylic functional groups on the organic polymer of the polymeric shell are blocked; wherein the detection probes are capable of generating a detection signal when present within the detection zone. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification