Detection of nucleic acids by type specific hybrid capture method
First Claim
Patent Images
1. A composition for detecting a viral nucleic acid comprising:
- at least two capture sequence probes, wherein each capture sequence probe consists of a nucleic acid sequence selected from the group consisting of SEQ ID NO;
1 through SEQ ID No;
29, SEQ ID NO;
50 through SEQ ID NO;
62, SEQ ID NO;
75 through SEQ ID NO;
109, SEQ ID NO;
152 through SEQ ID NO;
154, and wherein at least one capture sequence probe is hybridized to a first region of the viral nucleic acid, andat least one blocker sequence probe, wherein the blocker sequence probe consists of a nucleic acid sequence selected from the group consisting of SEQ ID NO;
30 through SEQ ID No;
49, SEQ ID NO;
63 through SEQ ID NO;
74, SEQ ID NO;
110 through SEQ ID NO;
151, SEQ ID NO;
155 through SEQ ID NO;
160, and wherein the blocker sequence probe is hybridized to at least one capture sequence probe that is not hybridized to the first region of viral nucleic acid.
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Abstract
Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid target sequences. The method produces DNA-RNA hybrids which can be detected by a variety of methods.
116 Citations
32 Claims
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1. A composition for detecting a viral nucleic acid comprising:
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at least two capture sequence probes, wherein each capture sequence probe consists of a nucleic acid sequence selected from the group consisting of SEQ ID NO;
1 through SEQ ID No;
29, SEQ ID NO;
50 through SEQ ID NO;
62, SEQ ID NO;
75 through SEQ ID NO;
109, SEQ ID NO;
152 through SEQ ID NO;
154, and wherein at least one capture sequence probe is hybridized to a first region of the viral nucleic acid, andat least one blocker sequence probe, wherein the blocker sequence probe consists of a nucleic acid sequence selected from the group consisting of SEQ ID NO;
30 through SEQ ID No;
49, SEQ ID NO;
63 through SEQ ID NO;
74, SEQ ID NO;
110 through SEQ ID NO;
151, SEQ ID NO;
155 through SEQ ID NO;
160, and wherein the blocker sequence probe is hybridized to at least one capture sequence probe that is not hybridized to the first region of viral nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 24, 25)
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23. A nucleic acid probe set comprising:
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(1) at least one capture sequence probe specific for a virus selected from the group consisting of;
HPV, HBV, HSV-1, and HSV-2, wherein the capture sequence probe consists of a sequence selected from the group consisting of;
SEQ ID NO;
1 through SEQ ID NO;
29, SEQ ID NO;
50 through SEQ ID NO;
62, SEQ ID NO;
75 through SEQ ID NO;
109, and SEQ ID NO;
152 through SEQ ID NO;
154; and(2) at least one blocker sequence probe specific for the capture sequence probe, wherein the blocker sequence probe consists of a sequence selected from the group consisting of;
SEQ ID NO;
30 ;
through SEQ ID NO;
49, SEQ ID NO;
63 through SEQ ID NO;
74, SEQ ID NO;
110 through SEQ ID NO;
151, and SEQ ID NO;
155 through SEQ ID NO;
160.
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26. A kit for detecting a viral nucleic acid comprising:
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(1) a capture sequence probe consists of a nucleic acid sequence selected from the group consisting of SEQ ID NO;
1 through SEQ ID NO;
29, SEQ ID NO;
50 through SEQ ID NO;
62, SEQ ID NO;
75 through SEQ ID NO;
109, SEQ ID NO;
152 through SEQ ID NO;
154, wherein the capture sequence probe is complementary to a first region of the viral nucleic acid; and(2) a blocker sequence probe consists of a nucleic acid sequence selected from the group consisting of SEQ ID NO;
30 through SEQ ID NO;
49, SEQ ID NO;
63 through SEQ ID NO;
74, SEQ ID NO;
110 through SEQ ID NO;
151, SEQ ID NO;
155 through SEQ ID NO;
160. - View Dependent Claims (27, 28, 29, 30, 31, 32)
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Specification