Process for enriching a population of sperm cells
First Claim
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1. A process for selectively decreasing the capacity of a subpopulation of sperm cells in a sperm cell dispersion to fertilize an egg, the process comprising:
- forming a dispersion of labeled sperm cells in a liquid, the liquid comprising a chemical agent that induces sperm immotility, wherein the presence, absence or amount of the label associated with a sperm cell indicates a genetic, proteomic, structural, or functional characteristic of a subpopulation of sperm cells in the dispersion, adjusting the density of the spermatozoa in the dispersion based upon variations among different species of mammals, variations among the mammals of a single species, or variations among different ejaculates of a single mammal;
optically inspecting the dispersion to identify individual sperm cells as members of the subpopulation;
determining the position of members of the subpopulation in the dispersion;
delivering a dose of energy to different positions within the dispersion to selectively decrease the capacity of members of the subpopulation to fertilize an egg without similarly affecting sperm cells at other positions in the dispersion, and returing the non-dosed subpopulation of sperm to an active state.
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Abstract
Processes for selectively enriching a population of viable sperm cells with respect to a characteristic without physically sorting the cells are disclosed. The cells contained in such an enriched population benefit from the advantage of not being subjected to a sorting process. Processes of inseminating a female mammal and processes of forming a sperm dispersion utilizing the processes of selectively enriching a population of viable sperm cells are also disclosed.
567 Citations
49 Claims
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1. A process for selectively decreasing the capacity of a subpopulation of sperm cells in a sperm cell dispersion to fertilize an egg, the process comprising:
- forming a dispersion of labeled sperm cells in a liquid, the liquid comprising a chemical agent that induces sperm immotility, wherein the presence, absence or amount of the label associated with a sperm cell indicates a genetic, proteomic, structural, or functional characteristic of a subpopulation of sperm cells in the dispersion, adjusting the density of the spermatozoa in the dispersion based upon variations among different species of mammals, variations among the mammals of a single species, or variations among different ejaculates of a single mammal;
optically inspecting the dispersion to identify individual sperm cells as members of the subpopulation;
determining the position of members of the subpopulation in the dispersion;
delivering a dose of energy to different positions within the dispersion to selectively decrease the capacity of members of the subpopulation to fertilize an egg without similarly affecting sperm cells at other positions in the dispersion, and returing the non-dosed subpopulation of sperm to an active state. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
- forming a dispersion of labeled sperm cells in a liquid, the liquid comprising a chemical agent that induces sperm immotility, wherein the presence, absence or amount of the label associated with a sperm cell indicates a genetic, proteomic, structural, or functional characteristic of a subpopulation of sperm cells in the dispersion, adjusting the density of the spermatozoa in the dispersion based upon variations among different species of mammals, variations among the mammals of a single species, or variations among different ejaculates of a single mammal;
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38. A process for inseminating a female mammal with an enriched sperm cell population, the process comprising:
- forming a dispersion of labeled sperm cells in a liquid, the liquid comprising a chemical agent that induces sperm immotility wherein the presence, absence or amount of the label associated with a sperm cell indicates a genetic, proteomic structural, or functional characteristic of a subpopulation of sperm cells in the dispersion, adjusting the density of the spermatozoa in the dispersion based upon variations among different species of mammals, variations among the mammals of a single species, or variations among different ejaculates of a single mammal;
optically inspecting the dispersion to identify individual sperm cells as members of the subpopulation;
determining the position of members of the subpopulation in the dispersion;
delivering a dose of energy to different positions within the dispersion to selectively decrease the capacity of members of the subpopulation to fertilize an egg without similarly affecting sperm cells at other positions in the dispersion;
returing the non-dosed subpopulation of sperm to an active state; and
thereafter inseminating a female mammal with the dispersion or a derivative thereof. - View Dependent Claims (39, 40, 41, 42)
- forming a dispersion of labeled sperm cells in a liquid, the liquid comprising a chemical agent that induces sperm immotility wherein the presence, absence or amount of the label associated with a sperm cell indicates a genetic, proteomic structural, or functional characteristic of a subpopulation of sperm cells in the dispersion, adjusting the density of the spermatozoa in the dispersion based upon variations among different species of mammals, variations among the mammals of a single species, or variations among different ejaculates of a single mammal;
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43. A process for in vitro fertilization, the process comprising:
- forming a dispersion of labeled sperm cells in a liquid, the liquid comprising a chemical agent that induces sperm immotility wherein the presence, absence or amount of the label associated with a sperm cell indicates a genetic, proteomic structural, or functional characteristic of a subpopulation of sperm cells in the dispersion, adjusting the density of the spermatozoa in the dispersion based upon variations among different species of mammals, variations among the mammals of a single species, or variations among different ejaculates of a single mammal;
optically inspecting the dispersion to identify individual sperm cells as members of the subpopulation;
determining the position of members of the subpopulation in the dispersion;
delivering a dose of energy to different positions within the dispersion to selectively decrease the capacity of members of the subpopulation to fertilize an egg without similarly affecting sperm cells at other positions in the dispersion;
returing the non-dosed subpopulation of sperm to an active state; and
thereafter fertilizing an egg, in vitro, using the dispersion or a derivative thereof. - View Dependent Claims (44, 45, 46, 47)
- forming a dispersion of labeled sperm cells in a liquid, the liquid comprising a chemical agent that induces sperm immotility wherein the presence, absence or amount of the label associated with a sperm cell indicates a genetic, proteomic structural, or functional characteristic of a subpopulation of sperm cells in the dispersion, adjusting the density of the spermatozoa in the dispersion based upon variations among different species of mammals, variations among the mammals of a single species, or variations among different ejaculates of a single mammal;
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48. A process for forming a frozen sperm dispersion, the process comprising:
- forming a dispersion of labeled sperm cells in a liquid, the liquid comprising a chemical agent that induces sperm immotility wherein the presence, absence or amount of the label associated with a sperm cell indicates a genetic, proteomic structural, or functional characteristic of a subpopulation of sperm cells in the dispersion, adjusting the density of the spermatozoa in the dispersion based upon variations among different species of mammals, variations among the mammals of a single species, or variations among different ejaculates of a single mammal;
optically inspecting the dispersion to identify individual sperm cells as members of the subpopulation;
determining the position of members of the subpopulation in the dispersion;
delivering a dose of energy to different positions within the dispersion to selectively decrease the capacity of members of the subpopulation to fertilize an egg without similarly affecting sperm cells at other positions in the dispersion; and
, thereafter cryopreserving the dispersion. - View Dependent Claims (49)
- forming a dispersion of labeled sperm cells in a liquid, the liquid comprising a chemical agent that induces sperm immotility wherein the presence, absence or amount of the label associated with a sperm cell indicates a genetic, proteomic structural, or functional characteristic of a subpopulation of sperm cells in the dispersion, adjusting the density of the spermatozoa in the dispersion based upon variations among different species of mammals, variations among the mammals of a single species, or variations among different ejaculates of a single mammal;
Specification