Nucleic acid amplification using microfluidic devices
First Claim
1. A microfluidic device, comprising(a) a substrate;
- (b) a first plurality of flow channels formed within the substrate, each of the first plurality of flow channels parallel to each other;
(c) a second plurality of flow channels formed within the substrate, each of the second plurality of flow channels parallel to each other and in fluid communication with an inlet, the second plurality of flow channels orthogonally intersecting the first plurality of flow channels to define an array of reaction chambers;
(d) isolation valves selectively actuatable to regulate solution flow to the reaction chambers, and to block flow between reaction chambers along at least one of;
a flow channel in the first plurality of flow channels;
ora flow channel in the second plurality of flow channels;
(e) a plurality of temperature regions located along each of the second plurality of flow channels; and
(f) a temperature controller operatively disposed to regulate temperature at one or more of the temperature regions.
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Accused Products
Abstract
The present invention provides microfluidic devices and methods using the same in various types of thermal cycling reactions. Certaom devices include a rotary microfluidic channel and a plurality of temperature regions at different locations along the rotary microfluidic channel at which temperature is regulated. Solution can be repeatedly passed through the temperature regions such that the solution is exposed to different temperatures. Other microfluidic devices include an array of reaction chambers formed by intersecting vertical and horizontal flow channels, with the ability to regulate temperature at the reaction chambers. The microfluidic devices can be used to conduct a number of different analyses, including various primer extension reactions and nucleic acid amplification reactions.
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Citations
47 Claims
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1. A microfluidic device, comprising
(a) a substrate; -
(b) a first plurality of flow channels formed within the substrate, each of the first plurality of flow channels parallel to each other; (c) a second plurality of flow channels formed within the substrate, each of the second plurality of flow channels parallel to each other and in fluid communication with an inlet, the second plurality of flow channels orthogonally intersecting the first plurality of flow channels to define an array of reaction chambers; (d) isolation valves selectively actuatable to regulate solution flow to the reaction chambers, and to block flow between reaction chambers along at least one of; a flow channel in the first plurality of flow channels;
ora flow channel in the second plurality of flow channels; (e) a plurality of temperature regions located along each of the second plurality of flow channels; and (f) a temperature controller operatively disposed to regulate temperature at one or more of the temperature regions. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A method for conducting an analysis, the method comprising:
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(a) providing a microfluidic device, comprising (i) a substrate; (ii) a first plurality of flow channels formed within the substrate, each of the first plurality of flow channels parallel to each other; (iii) a second plurality of flow channels formed within the substrate, each of the second plurality of flow channels parallel to each other and in fluid communication with an inlet, the second plurality of flow channels orthogonally intersecting the first plurality of flow channels to define an array of reaction chambers; and (iv) isolation valves selectively actuatable to regulate solution flow to the reaction chambers, and to block flow between reaction chambers along at least one of; a flow channel in the first plurality of flow channels;
ora flow channel in the second plurality of flow channels; (b) introducing a sample and one or more reactants into the reaction chambers by selective actuation of one or more of the isolation valves, whereby reaction between the sample and the one or more reactants can occur; and (c) heating regions of the microfluidic device to promote reaction between the sample and the one or more reactants within the reaction chambers. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46)
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47. A microfluidic device, comprising
(a) a substrate (b) a first plurality of flow channels formed within the substrate, each of the first plurality of flow channels parallel to each other; -
(c) a second plurality of flow channels formed within the substrate, each of the second plurality of flow channels parallel to each other and in fluid communication with an inlet, the second plurality of flow channels orthogonally intersecting the first plurality of flow channels to define an array of reaction chambers; and (c) isolation valves selectively actuatable to regulate solution flow to the reaction chambers, and to block flow between reaction chambers alone at least one of; a flow channel in the first plurality of flow channels;
ora flow channel in the second plurality of flow channels; wherein the isolation valves comprise a first and second isolation valve that have differing activation thresholds.
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Specification