Biomolecules having multiple attachment moieties for binding to a substrate surface
First Claim
1. A method of binding a biomolecule to a substrate comprising the steps of:
- providing a biomolecule;
contacting the biomolecule with a branched linking moiety to form a branched linking structure that is adapted to couple with the substrate, wherein the branched linking structure has multiple reactive groups; and
contacting the branched linking structure with the substrate to form a coupled substrate binding structure, where the biomolecule is bound to the substrate through multiple linkages.
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Accused Products
Abstract
Methods of binding biomolecules to a substrate are provided that include contacting the biomolecule with a branched linking moiety to form a branched linking structure. The branched linking structure is then contacted with a binding moiety on the substrate to form a coupled substrate binding structure, thereby binding the biomolecule to the substrate. The biomolecule may contain a Lewis base or a nucleophile to react with a Lewis acid or electrophile in the branched linking moiety. Alternatively, the biomolecule may contain a Lewis acid or electrophile that can react with a Lewis base or nucleophile in the branched linking moiety. Additionally, the biomolecule can be bound to the substrate through a covalent or non-covalent bond.
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Citations
15 Claims
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1. A method of binding a biomolecule to a substrate comprising the steps of:
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providing a biomolecule; contacting the biomolecule with a branched linking moiety to form a branched linking structure that is adapted to couple with the substrate, wherein the branched linking structure has multiple reactive groups; and contacting the branched linking structure with the substrate to form a coupled substrate binding structure, where the biomolecule is bound to the substrate through multiple linkages. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification