Methods of labelling polynucleotides with dibenzorhodamine dyes
First Claim
1. A method of identifying polynucleotide classes comprising the steps of:
- providing multiple classes of polynucleotides distributed among locations in a spatially addressable hybridization array;
wherein a first class of polynucleotide comprises a dibenzorhodamine dye having a structure of the formula;
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Abstract
Dibenzorhodamine compounds having the structure
are disclosed, including nitrogen- and aryl-substituted forms thereof. In addition, two intermediates useful for synthesizing such compounds are disclosed, a first intermediate having the structure
including nitrogen- and aryl-substituted forms thereof, and a second intermediate having the structure
including nitrogen- and aryl-substituted forms thereof, wherein substituents at positions C-14 to C18 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylic acid, sulfonic acid, —CH2OH, alkoxy, phenoxy, linking group, and substituted forms thereof. The invention further includes energy transfer dyes comprising the dibenzorhodamine compounds, nucleosides labeled with the dibenzorhodamine compounds, and nucleic acid analysis methods employing the dibenzorhodamine compounds.
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Citations
55 Claims
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1. A method of identifying polynucleotide classes comprising the steps of:
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providing multiple classes of polynucleotides distributed among locations in a spatially addressable hybridization array; wherein a first class of polynucleotide comprises a dibenzorhodamine dye having a structure of the formula; - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A method of identifying polynucleotide classes comprising the steps of:
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providing multiple classes of polynucleotides distributed among locations in a spatially addressable hybridization array; wherein a first class of polynucleotide comprises an energy transfer dye comprising; a donor dye configured to absorb light at a first wavelength and emit excitation energy in response; an acceptor dye configured to absorb the excitation energy emitted by the donor dye and fluoresce at a second wavelength in response; and a linker for linking the donor dye and the acceptor dye, the linker serving to facilitate the efficient transfer of energy between the donor dye and the acceptor dye; wherein at least one of the donor dye and acceptor dye is a dibenzorhodamine dye having a structure of the formula; - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50)
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51. A method of identifying polynucleotide classes comprising the steps of:
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providing multiple classes of polynucleotides distributed among locations in a spatially addressable hybridization array; wherein a first class of polynucleotide comprises a dibenzorhodamine dye having a structure of the formula; - View Dependent Claims (52, 53, 54, 55)
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Specification