Plant seed comprising vernolic acid

• US 7,834,248 B2
• Filed: 01/30/2007
• Issued: 11/16/2010
• Est. Priority Date: 04/15/1997
• Status: Expired due to Fees

• Current Assignee: Commonwealth Scientific & Industrial Research Organisation (Commonwealth Of Australia As Represented By Department Of Industry, Innovation, Climate Change, Science, Research And Tertiary Education)
• Original Assignee: Commonwealth Scientific & Industrial Research Organisation (Commonwealth Of Australia As Represented By Department Of Industry, Innovation, Climate Change, Science, Research And Tertiary Education)
• Inventors: Green, Allan, Lenman, Marit, Stymne, Sten, Singh, Surinder
• Primary Examiner(s): McElwain; Elizabeth F
• Application Number:
  US11/699,817
• Publication Number:
  US 20070209092A1
• Time in Patent Office: 1,386 Days
• Field of Search: None
• US Class Current: 800/298
• CPC Class Codes: C12N 15/8247 involving modified lipid metabolism, e.g. seed oil composition
• CPC Class Codes: C12N 9/0004 Oxidoreductases (1.)
• CPC Class Codes: C12N 9/0071 acting on paired donors with incorporation of molecular oxygen (1.14)
• CPC Class Codes: C12P 7/6409 Fatty acids
• CPC Class Codes: C12P 7/6472 containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Abstract

The present invention relates generally to novel genetic sequences that encode fatty acid epoxygenase enzymes, in particular fatty acid Δ12-epoxygenase enzymes from plants that are mixed function monooxygenase enzymes. More particularly, the present invention exemplifies cDNA sequences from Crepis spp. and Vernonia galamensis that encode fatty acid Δ12-epoxygenases. The genetic sequences of the present invention provide the means by which fatty acid metabolism may be altered or manipulated in organisms, such as, for example, yeasts, moulds, bacteria, insects, birds, mammals and plants, and more particularly in plants. The invention also extends to genetically modified oil-accumulating organisms transformed with the subject genetic sequences and to the oils derived therefrom. The oils thus produced provide the means for the cost-effective raw materials for use in the efficient production of coatings, resins, glues, plastics, surfactants and lubricants.

Claims

1. A plant seed comprising 12,13-epoxy-9-octadecenoic acid at a level of greater than 0.7% (w/w) of total seed fatty acid content in the plant seed, wherein the plant is Arabidopsis thaliana, flax, oilseed rape, sunflower, corn, safflower, soybean, sesame, cottonseed, peanut, olive or oil palm.

2. The plant seed of claim 1, wherein the plant is Arabidopsis thaliana.

3. The plant seed of claim 1, wherein the plant is flax, sunflower, corn, or safflower.

4. The plant seed of claim 3, wherein the plant is flax and the flax is Linum usitatissimum.

5. The plant seed of claim 3, wherein the plant is a flax plant and the plant seed contains less than 2% linolenic acid.

6. The plant seed of claim 1, wherein the plant seed further comprises 12,13-epoxy-9,15-octadecadienoic acid.

7. The plant seed of claim 3, wherein the plant seed further comprises 12,13-epoxy-9,15-octadecadienoic acid.

8. The plant seed of claim 4, wherein the plant seed further comprises 12,13-epoxy-9,15-octadecadienoic acid.

9. A process for producing oil containing epoxy fatty acids comprising obtaining as a starting material the plant seed of claim 1, and isolating from the plant seed the oil, thereby producing the oil containing epoxy fatty acids.

10. The process of claim 9, wherein the plant is Arabidopsis thaliana.

11. The process of claim 9, wherein the plant is flax, sunflower, corn, or safflower.

12. The process of claim 11, wherein the plant is flax and the flax is Linum usitatissimum.

13. The process of claim 11, wherein the plant is a flax plant and the plant seed contains less than 2% linolenic acid.

14. The process of claim 9, wherein the plant seed further comprises 12,13-epoxy-9,15-octadecadienoic acid.

15. A process for producing an epoxy fatty acid comprising obtaining as a starting material the plant seed of claim 1, and isolating from the plant seed the epoxy fatty acid, thereby producing the epoxy fatty acid.

16. The process of claim 15, wherein the plant is Arabidopsis thaliana.

17. The process of claim 15, wherein the plant is flax, sunflower, corn, or safflower.

18. The process of claim 17, wherein the plant is flax and the flax is Linum usitatissimum.

19. The process of claim 17, wherein the plant is a flax plant and the plant seed contains less than 2% linolenic acid.

20. The process of claim 15, wherein the plant seed further comprises 12,13-epoxy-9,15-octadecadienoic acid.