Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
DCFirst Claim
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1. A Brassica or Arabidopsis plant cell comprisingdocosapentaenoic acid and docosahexaenoic acid in an esterified form as part of triacylglycerols in the plant cell;
- a polynucleotide which encodes a Δ
5 elongase operably linked to a promoter which directs expression of the polynucleotide in the plant cell;
a polynucleotide which encodes a Δ
4 desaturase operably linked to a promoter which directs expression of the polynucleotide in the plant cell;
a desaturase which desaturates an acyl-CoA substrate in the plant cell; and
a total fatty acid content in the plant cell which includes 2.5% (w/w) C20 ω
3 long-chain polyunsaturated fatty acids (LC-PUFAs) and 0.1% (w/w) docosapentaenoic acid.
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Abstract
The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.
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Citations
16 Claims
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1. A Brassica or Arabidopsis plant cell comprising
docosapentaenoic acid and docosahexaenoic acid in an esterified form as part of triacylglycerols in the plant cell; -
a polynucleotide which encodes a Δ
5 elongase operably linked to a promoter which directs expression of the polynucleotide in the plant cell;a polynucleotide which encodes a Δ
4 desaturase operably linked to a promoter which directs expression of the polynucleotide in the plant cell;a desaturase which desaturates an acyl-CoA substrate in the plant cell; and a total fatty acid content in the plant cell which includes 2.5% (w/w) C20 ω
3 long-chain polyunsaturated fatty acids (LC-PUFAs) and 0.1% (w/w) docosapentaenoic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification