Methods and apparatus for isolating platelets from blood
First Claim
1. A method for separating platelets from blood for application to a patient in a medical treatment, comprising:
- placing the blood in a container having a sidewall and a bottom at least partially defining a volume;
positioning a float that has a surface that defines a collection area within the volume of the container;
providing a force on the float and the blood;
causing the float to move from near the bottom of the container within the volume due at least in part to the force provided on the float;
sedimenting platelets from the blood placed in the container on a thin layer of erythrocytes resting on the surface of the float in the container within the volume; and
accessing the volume through at least one of two access ports passing through a top wall at a top end of the container.
1 Assignment
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Accused Products
Abstract
A platelet collection device comprising a centrifugal spin-separator container with a cavity having a longitudinal inner surface. A float in the cavity has a base, a platelet collection surface above the base, an outer surface. The float density is below the density of erythrocytes and above the density of plasma. The platelet collection surface has a position on the float which places it below the level of platelets when the float is suspended in separated blood. During centrifugation, a layer of platelets or buffy coat collects closely adjacent the platelet collection surface. Movement of a float having a density greater than whole blood through the sedimenting erythrocytes releases entrapped platelets, increasing the platelet yield.
430 Citations
17 Claims
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1. A method for separating platelets from blood for application to a patient in a medical treatment, comprising:
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placing the blood in a container having a sidewall and a bottom at least partially defining a volume; positioning a float that has a surface that defines a collection area within the volume of the container; providing a force on the float and the blood; causing the float to move from near the bottom of the container within the volume due at least in part to the force provided on the float; sedimenting platelets from the blood placed in the container on a thin layer of erythrocytes resting on the surface of the float in the container within the volume; and accessing the volume through at least one of two access ports passing through a top wall at a top end of the container. - View Dependent Claims (2, 3, 4, 6, 7)
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5. A method for separating platelets from blood for application to a patient in a medical treatment, comprising:
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placing the blood in a container having a sidewall and a bottom at least partially defining a volume; sedimenting platelets from the blood placed in the container on a thin layer of erythrocytes resting on a surface in the container within the volume; accessing the volume through at least one of two access ports passing through a top wall at a top of the container; positioning a float within the volume of the container; providing a force on the float and the blood; causing the float to move within the volume due at least in part to the force provided on the float; and defining a collection area with the float, wherein defining a collection area includes;
providing an outer rim of the float having a first height and providing a top wall of the float defining a depressed center having a point below the first height of the outer rim;wherein sedimenting the platelets includes sedimenting the erythrocytes near the surface of the float near the collection area on the thin layer of erythrocytes.
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8. A method for separating platelets from a blood volume for application to a patient in a medical treatment, comprising:
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providing a container having a sidewall and a bottom at least partially defining a container volume; positioning a first member within the container volume; providing a cap with at least two access ports in the cap on the container; placing the blood volume in the container volume; providing a force on the blood volume to move the first member; allowing a portion of the blood volume to pass through an opening in the first member; separating erythrocytes from the blood volume; forming the first member to have a density to allow the first member to move from near the bottom of the container to a position near the erythrocytes separated from the blood volume to allow formation of a thin layer of erythrocytes on a surface of the first member; and sedimenting platelets on the thin layer of erythrocytes resting on the surface; and accessing the sedimented platelets through at least one of the two access ports in the cap. - View Dependent Claims (9, 14)
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10. A method for separating platelets from a blood volume for application to a patient in a medical treatment, comprising:
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providing a container having a sidewall and a bottom at least partially defining a container volume; positioning a first member within the container volume; positioning a second member in the container; placing the blood volume in the container volume; providing a force on the blood volume to move the first member; allowing a portion of the blood volume to pass through an opening in the first member; moving the second member through the portion of the blood; separating erythrocytes from the blood volume; forming the first member to have a density to allow the first member to move from near the bottom of the container to a position near the erythrocytes separated from the blood volume to allow formation of a thin layer of erythrocytes on a surface of the first member; sedimenting platelets on the thin layer of erythrocytes resting on the surface; orienting the container in a selected manner; and moving a limited portion of the blood volume to a first section of the container volume separated from a second section of the container volume. - View Dependent Claims (11, 12, 13)
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15. A method for separating platelets from blood for application to a patient in a medical treatment, comprising:
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providing a container; positioning the blood in the container by delivering the blood into the container through a first port in a first end wall of the provided container; providing a force to separate the blood; moving a float from a first position to a second position to assist in separating the blood; after moving the float to the second position, moving at least a portion of the separated blood to a selected section of the container away from the float in the second position; moving a member in the provided container through at least a portion of the blood relative to the float; and withdrawing a portion of the separated blood from the container through a second port in the first end wall of the provided container; wherein withdrawing a portion of the separated blood from the container through the second port includes withdrawing the portion from a layer from between the float and the member. - View Dependent Claims (16)
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17. A method for separating platelets from blood for application to a patient in a medical treatment, comprising:
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providing a container; positioning the blood in the container; providing a force to separate the blood; moving a float from a first position to a second position to assist in separating the blood; and after moving the float to the second position, moving at least a portion of the separated blood to a selected section of the container away from the float in the second position; wherein moving at least a portion of the separated blood includes moving a member in the provided container and through at least a portion of the blood after separating the blood.
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Specification