Method and apparatus for isolating and purifying nucleic acid using a single surface

• US 7,838,036 B2
• Filed: 03/03/2010
• Issued: 11/23/2010
• Est. Priority Date: 09/25/2006
• Status: Active Grant

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Yoo, Chang-eun, Jeong, Sung-young, Kim, Young-rok
• Primary Examiner(s): Riley; Jezia
• Application Number:
  US12/716,695
• Publication Number:
  US 20100160585A1
• Time in Patent Office: 265 Days
• Field of Search: 424/489, 424/490, 435/5, 435/6, 435/7.1, 435/7.2, 536/23.1
• US Class Current: 424/489
• CPC Class Codes: B01D 15/1807 using counter-currents, e.g. fluidised beds
• CPC Class Codes: B01J 20/28009 Magnetic properties
• CPC Class Codes: B01J 20/3204 Inorganic carriers, supports or substrates
• CPC Class Codes: B01J 20/321 consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3212 consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3219 involving a particular spacer or linking group, e.g. for attaching an active group
• CPC Class Codes: B01J 20/327 Polymers obtained by reactions involving only carbon to carbon unsaturated bonds
• CPC Class Codes: C08F 20/02 Monocarboxylic acids having less than ten carbon atoms, Derivatives thereof
• CPC Class Codes: C08F 26/06 by a heterocyclic ring containing nitrogen
• CPC Class Codes: C12N 1/066 by physical methods
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Abstract

Provided is a method of isolating nucleic acid from cells using a single surface, wherein a compound represented by Formula 1 is bound to the surface. Also provided are an apparatus for isolation of nucleic acids, and a bead for isolating nucleic acids.

Claims

1. A bead for isolating nucleic acid from cells, comprising

a material represented by Formula 1 below bound to the surface of the bead:

2. The bead of claim 1, wherein R₁₀ is a pyridinyl group or an imidazolyl group.

3. The bead of claim 1, wherein R₁ and R₅ are each a hydroxyl group.

4. A method of making a bead surface for isolating nucleic acid from cells, comprising:

reacting a bead with a compound to introduce a hydrophobic functional group, wherein the hydrophobic functional group is a substituted or unsubstituted C4-C20 alkyl group, a substituted or unsubstituted C4-C20 alkoxy group, a substituted or unsubstituted C4-C20 alkenyl group, a substituted or unsubstituted C6-C30 aryl group, or a substituted or unsubstituted C6-C30 aryloxy group;

reacting the bead with a second compound to introduce a charge switching moiety, wherein the charge switching moiety is a nitrogen-containing heteroaryl or heterocyclic group of 3-30 carbon atoms.