Apparatus and method for parallel flow ion mobility spectrometry combined with mass spectrometry

• US 7,838,826 B1
• Filed: 08/07/2008
• Issued: 11/23/2010
• Est. Priority Date: 08/07/2008
• Status: Active Grant

• Current Assignee: Bruker Daltonics, Inc. (Bruker Corporation)
• Original Assignee: Bruker Daltonics, Inc. (Bruker Corporation)
• Inventors: Park, Melvin A.
• Primary Examiner(s): Wells; Nikita
• Application Number:
  US12/187,924
• Time in Patent Office: 838 Days
• Field of Search: 250/288, 250/287, 250/281, 250/282, 250/283, 250/423.R, 250/396.R
• US Class Current: 250/288
• CPC Class Codes: G01N 27/623 combined with mass spectrometry
• CPC Class Codes: H01J 49/004 Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn
• CPC Class Codes: H01J 49/066 Ion funnels

Abstract

Analyte ions entrained in a carrier gas are analyzed by parallel flow ion mobility spectrometry prior to analysis by a mass analyzer. An extended ion funnel is located in the vacuum system of the mass analyzer and has an ion focusing section and an ion mobility analyzing section. The carrier gas together with entrained ions is introduced into the ion focusing section where the ions are focused to the axis of the funnel by applied RF voltages. In the ion mobility section, the action of an RF quadrupolar field, the movement of the carrier gas and axial DC field, separates the ions on the basis of their mobilities. The mobility separated ions are released into the mass analyzer where the ions may be further separated on the basis of mass.

Claims

1. A combination spectrometer that analyzes ions by parallel flow ion mobility and by mass comprising:

a mechanism that entrains the sample ions in a carrier gas;

a mass spectrometer having a vacuum system with an extended ion funnel and a mass analyzer located inside the vacuum system;

a mechanism that introduces the sample ions and the carrier gas into an entrance end of the extended ion funnel, the vacuum system drawing the carrier gas and the sample ions through the extended ion funnel to an exit end of the extended ion funnel and towards the mass analyzer;

wherein the extended ion funnel is constructed with a plurality of segmented electrodes that are connected to an RF voltage source so that an ion focusing section is formed inside the extended ion funnel near the entrance end and an ion mobility analyzing section with an RF containment field is formed inside the extended ion funnel near the exit end and the sample ions are separated by ion mobility as they move through the extended ion funnel.

2. The apparatus of claim 1 wherein the mechanism that introduces the sample ions and the carrier gas into the extended ion funnel comprises an ion focusing section of the extended ion funnel that is connected to the RF voltage source so that a dipolar focusing RF field is generated therein.

3. The apparatus of claim 2 wherein the ion focusing section has an axis and is comprised of a plurality of segmented electrodes positioned perpendicular to, and spaced along, the axis.

4. The apparatus of claim 3 wherein spaces between adjacent electrodes comprising the ion focusing section are open so that the carrier gas may pass between the adjacent electrodes.

5. The apparatus of claim 4 wherein each of the plurality of electrodes comprising the ion focusing section has a central circular opening and wherein the plurality of electrodes have diameters that vary as a function of position along the axis.

6. The apparatus of claim 5 wherein the diameters of central openings of electrodes comprising the ion focusing section which are near the ion mobility analyzing section are smaller than the diameters of central openings of electrodes at which the ions and the carrier gas enter the ion focusing section.

7. The apparatus of claim 1 further comprising an ion source that generates the sample ions utilizing one of the group consisting of electrospray ionization, atmospheric pressure chemical ionization, atmospheric pressure photoionization, laser desorption ionization, matrix assisted laser desorption ionization, and desorption electrospray ionization.

8. The apparatus of claim 1 wherein the carrier gas is one of the group consisting of N₂, O₂, CO₂, H₂O, Ar, He, CH₄, and mixtures thereof.

9. The apparatus of claim 1 wherein the extended ion funnel has an ion focusing section that follows the ion mobility analyzing section in the direction of ion motion.

10. The apparatus of claim 1 wherein the ion mobility analyzing section has an axis and is comprised of a plurality of segmented electrodes positioned perpendicular to, and spaced along, the axis.

11. The apparatus of claim 10 wherein each of the plurality of electrodes comprising the analyzing section has a central circular opening and wherein the central openings of all of the electrodes have a same diameter.

12. The apparatus of claim 10 wherein each of the plurality of electrodes comprising the analyzing section has a central circular opening and wherein the plurality of electrodes have diameters that vary as a function of position along the axis.

13. The apparatus of claim 10 wherein gaskets are placed between adjacent electrodes comprising the analyzing section such that the electrodes and the gaskets form a gas tight channel along the axis.

14. The apparatus of claim 10 wherein each of the plurality of segmented electrodes comprising the analyzing section has four segments.

15. The apparatus of claim 10 wherein each of the plurality of segmented electrodes comprising the analyzing section has six segments.

16. The apparatus of claim 10 wherein each of the plurality of segmented electrodes comprising the analyzing section has one segment.

17. The apparatus of claim 1 wherein the mass analyzer comprises one of the group consisting of time-of-flight mass analyzers, quadrupole filters, Paul ion traps, quadrupole linear ion traps, ion cyclotron resonance mass analyzers, and orbitrap mass analyzers.

18. The apparatus of claim 1 wherein each of the plurality of segmented electrodes has four segments.

19. The apparatus of claim 1 wherein each of the plurality of segmented electrodes has one segment.

20. The apparatus of claim 1 further comprising a deflection electrode located in the vacuum system, positioned adjacent to the mechanism that introduces the sample ions and the carrier gas into the extended ion funnel and connected to a voltage source for directing the sample ions into the mechanism that introduces the sample ions and the carrier gas into the extended ion funnel.

21. The apparatus of claim 1 further comprising a sample located in the vacuum system, a laser located outside of the vacuum system, and a window in the vacuum system for allowing laser light generated by the laser to impinge on the sample.

22. A method for analyzing sample ions by parallel flow ion mobility spectrometry in combination with a mass spectrometer having,

an ion source,

a vacuum system having an extended ion funnel and a mass analyzer located therein,

a deflection electrode located in the vacuum system that introduces the sample ions and the carrier gas into an entrance end of the extended ion funnel, the vacuum system drawing the carrier gas and the sample ions through the extended ion funnel to an exit end of the extended ion funnel and towards the mass analyzer,

an extended ion funnel located in the vacuum system and having a plurality of segmented electrodes that are connected to voltage sources so that an ion focusing section is formed inside the extended ion funnel near the entrance end and an ion mobility analyzing section with an axis and an RF containment field is formed inside the extended ion funnel near the exit end and the sample ions are separated by ion mobility as they move through the extended ion funnel, and

an ion detector,

the method comprising:

(a) forming a DC barrier in the ion mobility analyzing section;

(b) applying an RF voltage to the ion mobility analyzing section for focusing ions towards the axis;

(c) generating ions in the ion source;

(d) entraining generated ions in a carrier gas;

(e) pulsing a group of entrained ions into extended ion funnel focusing section by applying a pulsed electrical potential to the deflection electrode;

(f) forcing the group of ions into ion mobility analyzing section by applying a first set of DC potentials to electrodes of the ion focusing section;

(g) preventing additional ions from entering the ion mobility analyzing section by applying a second set of DC potentials to the deflection electrode and the electrodes of the ion focusing section;

(h) inducing a carrier gas flow through the ion mobility analyzing section away from the ion focusing section with a vacuum pump in the vacuum system;

(i) gradually reducing the DC barrier formed in step (a) to allow the carrier gas flow to push ions from the group of ions over the DC barrier in order of the ions'"'"' mobilities;

(j) focusing ions that have been pushed over the DC barrier with the second ion focusing section; and

(k) detecting ions that have been focused in step (j) with the ion detector.