Microchip for analysis, analysis system having the same, and analysis method

• US 7,842,240 B2
• Filed: 05/03/2005
• Issued: 11/30/2010
• Est. Priority Date: 05/06/2004
• Status: Active Grant

• Current Assignee: Seiko Instruments Incorporated (Seiko Group)
• Original Assignee: Seiko Instruments Incorporated (Seiko Group)
• Inventors: Shinogi, Masataka, Yamamoto, Minao
• Primary Examiner(s): JARRETT, LORE RAMILLANO
• Application Number:
  US11/121,321
• Publication Number:
  US 20050255000A1
• Time in Patent Office: 2,037 Days
• Field of Search: 422/68.1, 422/103, 422/107, 422/110
• US Class Current: 422/68.1
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/141 Preventing contamination, tampering
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/1805 Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites

Abstract

A microchip has a substrate on which there are formed a reaction bath section, a first flow channel to which are connected a supply channel that supplies a buffer solution and the reaction bath section, a second flow channel to which are connected a supply channel that supplies a sample solution containing a first substance and a channel that discharges the sample and buffer solutions to an exterior of the substrate, and a connecting channel connecting the first flow channel to the second flow channel. The reaction bath section receives the first and second substances so that the first and second substances react in the reaction bath section. The first and second flow channels are provided with valves disposed relative to the connecting channel such that selective opening and closing of the valves causes the buffer solution to flow first into the reaction bath section at a preselected speed and the sample solution to flow second into the reaction bath section at the preselected speed to cause the first and second substances to react in the reaction bath section.

Claims

1. A microchip for conducting an analysis, the microchip comprising:

a substrate;

a sample solution supply channel that is formed in the substrate and that supplies a sample solution containing a first substance;

a reaction bath section that is formed in the substrate and that receives the first substance and a second substance that reacts with the first substance in the reaction bath section;

a buffer solution supply channel that is formed in the substrate and that supplies a buffer solution which does not substantially react with the first and second substances;

a waste solution channel that is formed in the substrate and that discharges the sample and buffer solutions to an exterior of the substrate;

a first flow channel formed in the substrate and to which the buffer solution supply channel and the reaction bath section are connected, the first flow channel having first and second valves controllable between open and closed states and disposed between the buffer solution supply channel and the reaction bath section;

a second flow channel formed in the substrate and to which the sample solution supply channel and the waste solution channel are connected, the second flow channel having third and fourth valves controllable between open and closed states and disposed between the sample solution supply channel and the waste solution channel; and

a connecting channel formed in the substrate and connecting the first flow channel to the second flow channel, the connecting channel being disposed at an upstream side of the reaction bath section and the waste solution channel and at a downstream side of the sample solution supply channel and the buffer solution supply channel, and the first and third valves being disposed at an upstream side of the connecting channel and the second and fourth valves being disposed at a downstream side of the connecting channel such that by selectively opening and closing the first, second, third and fourth valves, the buffer solution from the buffer solution supply channel flows first into the reaction bath section at a preselected speed followed by the flow of the sample solution from the sample solution supply channel into the reaction bath section, via the connecting channel, at the preselected speed so that the first and second substances react in the reaction bath section.

2. A microchip for conducting an analysis according to claim 1;

wherein in the open states of the first and fourth valves and the closed state of the second and third valves, the buffer solution flows from the buffer solution supply channel to the waste solution channel.

3. A microchip for conducting an analysis according to claim 1;

wherein the sample solution supply channel is provided with a sample solution introducing port through which the sample solution is dropped and supplied, and the buffer solution supply channel is provided with a buffer solution introducing port through which the buffer solution is dropped and supplied.

4. A microchip for conducting an analysis according to claim 1;

wherein the waste solution channel is connected to a waste solution tank which is provided exteriorly of the substrate.

5. A microchip for conducting an analysis according to claim 4;

wherein the reaction bath section is connected to the waste solution tank.

6. A microchip for conducting an analysis according to claim 4;

wherein the waste solution tank is connected to a pump which draws a fluid from at least one of the waste solution channel and the reaction bath section.

7. A microchip for conducting an analysis according to claim 1;

further comprising a temperature adjusting section provided in the substrate to adjust a temperature of each of the buffer solution and the sample solution.

8. A microchip for conducting an analysis according to claim 7;

wherein the temperature adjusting section is disposed upstream of the reaction bath section and downstream of the first and second valves.

9. A microchip for conducting an analysis according to claim 7;

wherein the temperature adjusting section is disposed in the reaction bath section.

10. A microchip for conducting an analysis according to claim 7;

wherein the temperature adjusting section is disposed upstream of the reaction bath section.

11. A microchip for conducting an analysis according to claim 10;

wherein the temperature adjusting section is provided downstream of the first and second valves in the first flow channel.

12. A microchip for conducting an analysis according to claim 1;

further comprising;

a third flow channel formed in the substrate and to which another buffer solution supply channel that is formed in the substrate and supplies the buffer solution and the reaction bath section are connected; and

a fourth flow channel formed in the substrate and to which a treatment solution supply channel that is formed in the substrate and that supplies a treatment solution and the waste solution channel are connected;

wherein the third flow channel is connected to the fourth flow channel by a second connecting channel formed in the substrate and disposed at an upstream side of the reaction bath section.

13. A microchip for conducting an analysis according to claim 12;

wherein the third flow channel comprises a flow channel which is branched from the buffer solution supply channel to which the first flow channel is connected to reach the reaction bath section.

14. A microchip for conducting an analysis according to claim 12;

wherein the second connecting channel comprises a flow channel via which the buffer solution flows from the another buffer solution supply channel to the waste solution channel and via which the treatment solution flows from the fourth flow channel to the reaction bath section.

15. A microchip for conducting an analysis according to claim 12;

wherein the fourth flow channel is provided with valves which are respectively disposed upstream and downstream of the second connecting channel, and the third flow channel is provided with valves which are respectively disposed upstream and downstream of a portion to which the another connecting channel is connected.

16. A microchip for conducting an analysis according to claim 12;

wherein a fifth flow channel for the treatment solution is formed in the substrate and connected to the fourth flow channel.

17. An analysis system comprising:

a microchip according to claim 1 for conducting an analysis;

a mounting section on which the microchip is mounted;

a solution transfer section by which at least the sample solution and the buffer solution flows in the microchip mounted on the mounting section;

a sensor section that detects a reaction between the first and second substances in the reaction bath section; and

an analysis section that analyzes the detection result from the sensor section.

18. An analysis system according to claim 17;

wherein the sensor section is built in the reaction bath section of the microchip.

19. An analysis system according to claim 17;

further comprising a computer that includes the analysis section and that controls opening and closing operations of the first, second, third and fourth valves in the first and second flow channels of the microchip.

20. A microchip for conducting an analysis according to claim 1;

wherein the sample solution supply channel is provided with a sample solution introducing port through which the sample solution is dropped and supplied, and the buffer solution supply channel is connected to a buffer solution tank which stores the buffer solution.

21. A microchip for conducting an analysis, the microchip comprising:

a substrate having a reaction bath section, a first flow channel to which are connected the reaction bath section and a buffer solution supply channel that supplies a buffer solution, a second flow channel to which are connected a sample solution supply channel that supplies a sample solution containing a first substance and a discharge channel that discharges the sample and buffer solutions to an exterior of the substrate, a connecting channel connecting the first flow channel to the second flow channel, first and second valves controllable between open and closed states and disposed in the first flow channel on opposite sides of the connecting channel and between the buffer solution supply channel and the reaction bath section, and third and fourth valves controllable between open and closed states and disposed in the second flow channel on opposite sides of the connecting channel and between the sample solution supply channel and the discharge channel, the reaction bath section being positioned relative to the connecting channel and the first, second, third, and fourth valves so that by selectively opening and closing the first, second, third and fourth valves, the buffer solution from the buffer solution supply channel flows first into the reaction bath section at a preselected speed followed by the flow of the sample solution from the sample solution supply channel into the reaction bath section, via the connecting channel, at the preselected speed so that the first substance reacts with a second substance in the reaction bath section.

22. A microchip according to claim 21;

wherein the substrate comprises a pair of plate members laminated over one another.

23. A microchip according to claim 21;

wherein the channel that discharges the sample and buffer solutions is connected to a waste solution tank provided exteriorly of the substrate.

24. A microchip according to claim 21;

further comprising a temperature adjusting section provided in the substrate to adjust a temperature of each of the buffer solution and the sample solution.

25. A microchip according to claim 24;

wherein the temperature adjusting section is disposed in the reaction bath section.

26. A microchip according to claim 24;

wherein the temperature adjusting section is disposed upstream of the reaction bath section.

27. A microchip according to claim 24;

wherein the temperature adjusting section is provided downstream of the valves provided in the first flow channel.

28. An analysis system comprising:

a microchip according to claim 21 for conducting an analysis;

a mounting section on which the microchip is mounted;

a solution transfer section by which at least the sample solution and the buffer solution flow in the microchip mounted on the mounting section;

a sensor section that detects a reaction between the first and second substances in the reaction bath section; and

an analysis section that analyzes the detection result from the sensor section.

29. An analysis system according to claim 28;

wherein the sensor section is built in the reaction bath section of the microchip.

30. An analysis system according to claim 28;

further comprising a computer that includes the analysis section and that controls opening and closing operations of the first, second, third and third valves in the first and second flow channels of the microchip.

31. A microchip for conducting an analysis, the microchip comprising:

a substrate having a reaction bath section, a first flow channel to which are connected the reaction bath section and a buffer solution supply channel that supplies a buffer solution, a second flow channel to which are connected a sample solution supply channel that supplies a sample solution containing a first substance and a discharge channel that discharges the sample and buffer solutions to an exterior of the substrate, a connecting channel connecting the first flow channel to the second flow channel, first and second valves disposed in the first flow channel on opposite sides of the connecting channel and between the buffer solution supply channel and the reaction bath section, and third and fourth valves disposed in the second flow channel on opposite sides of the connecting channel and between the sample solution supply channel and the discharge channel, the first, second, third and fourth valves being controllable between open and closed states to extract air from the first flow channel, second flow channel and connecting channel so that by subsequently selectively opening and closing the first, second, third and fourth valves, the buffer solution from the buffer solution supply channel flows first into the reaction bath section at a preselected speed followed by the flow of the sample solution from the sample solution supply channel into the reaction bath section, via the connecting channel, at the preselected speed so that the first substance reacts with a second substance in the reaction bath section.

32. A microchip for conducting an analysis according to claim 31;

further comprising a temperature adjusting section provided in the substrate to adjust a temperature of each of the buffer solution and the sample solution, the temperature adjusting section being disposed upstream of the reaction bath section and downstream of the first and second valves.

33. An analysis system comprising:

a microchip according to claim 31 for conducting an analysis;

a mounting section on which the microchip is mounted;

a solution transfer section by which at least the sample solution and the buffer solution flows in the microchip mounted on the mounting section;

a sensor section that detects a reaction between the first and second substances in the reaction bath section; and

an analysis section that analyzes the detection result from the sensor section.