Method for increasing the content of polyunsaturated long-chained fatty acids in transgenic organisms
First Claim
1. A method for producing oils and/or lipids having a high content of polyunsaturated fatty acids containing at least two double bonds in a transgenic organism comprising:
- a) introducing at least one nucleic acid sequence coding for a polypeptide exhibiting a phospholipase A2 activity into an organism,b) cultivating the organism.
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Abstract
The present invention relates to a method for increasing the content of polyunsaturated long-chain fatty acids in an organism by introducing nucleic acids coding for polypeptides or proteins exhibiting a phospholipase, ketoacyl-CoA reductase and/or dehydratase activity. The present invention also provides nucleic acid sequences coding for polypeptides with enzyme activities of a phospholipase, ketoacyl-CoA reductase and/or dehydratase, and nucleic acid constructs, vectors and organisms containing the nucleic acid sequences according to the present invention. A further part of the present invention relates to oils, lipids and/or fatty acids produced according to the method of the present invention and the use thereof. Furthermore, the present invention relates to unsaturated fatty acids and triglycerides having an increased content of unsaturated fatty acids and the use thereof.
47 Citations
23 Claims
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1. A method for producing oils and/or lipids having a high content of polyunsaturated fatty acids containing at least two double bonds in a transgenic organism comprising:
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a) introducing at least one nucleic acid sequence coding for a polypeptide exhibiting a phospholipase A2 activity into an organism, b) cultivating the organism. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. An isolated nucleic acid molecule comprising a nucleic acid sequence coding for a polypeptide or a protein exhibiting phospholipase A2 activity, characterized in that the nucleic acid sequence is selected from the group consisting of:
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a) a nucleic acid sequence having the sequence depicted in SEQ ID NO;
1,b) a nucleic acid sequence which encodes the amino acid sequence depicted in SEQ ID NO;
2, andc) a derivative of the nucleic acid sequence depicted in SEQ ID NO;
1, which codes for a polypeptide or a protein having at least 70% homology at the amino acid level to SEQ ID NO;
2 and exhibiting a phospholipase A2 activity. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification