Polynucleotides for use as tags and tag complements, manufacture and use thereof
First Claim
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1. An oligonucleotide tag or tag complement for use in a multiplex assay, wherein the tag or tag complement is selected from the group of oligonucleotides consisting of:
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Abstract
A family of minimally cross-hybridizing nucleotide sequences, methods of use, etc. A specific family of 210 24mers is described.
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Citations
28 Claims
- 1. An oligonucleotide tag or tag complement for use in a multiplex assay, wherein the tag or tag complement is selected from the group of oligonucleotides consisting of:
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6. A nucleic acid tag or tag complement comprising an oligonucleotide selected from the group of oligonucleotides consisting of:
- SEQ ID NO;
68, SEQ ID NO;
69, SEQ ID NO;
70, SEQ ID NO;
72, SEQ ID NO;
74, SEQ ID NO;
75, SEQ ID NO;
77, SEQ ID NO;
79, SEQ ID NO;
80, and SEQ ID NO;
81, or a sequence complementary thereto, wherein the oligonucleotide is attached to a solid support. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
- SEQ ID NO;
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23. A combination of oligonucleotide tags or tag complements for use in a multiplexed assay comprising at least one oligonucleotide set forth in SEQ ID NOs:
- 68-70, 72, 74, 75, 77, or 79-81 and an oligonucleotide selected from the group consisting of SEQ ID NOs;
1-210, wherein the combination comprises at least two different oligonucleotides set forth in SEQ ID NOs;
1-210. - View Dependent Claims (24, 25, 26, 27)
- 68-70, 72, 74, 75, 77, or 79-81 and an oligonucleotide selected from the group consisting of SEQ ID NOs;
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28. A combination comprising a plurality of oligonucleotide tags or tag complements for use in a multiplexed assay, wherein the plurality of tags or tag complements comprise SEQ ID NOs:
- 68-70, 72, 74, 75, 77, and 79-81 and sequences complementary thereto.
Specification