Tissue engineering in vivo with vascularized scaffolds

• US 7,846,728 B2
• Filed: 10/09/2007
• Issued: 12/07/2010
• Est. Priority Date: 10/13/2006
• Status: Expired due to Fees

• Current Assignee: Biostruxs LLC
• Original Assignee: Biostruxs LLC
• Inventors: Watson, James P, Brooks, Mai Nguyen
• Primary Examiner(s): Ton; Thaian N
• Assistant Examiner(s): NOBLE, MARCIA STEPHENS
• Application Number:
  US11/973,812
• Publication Number:
  US 20080090292A1
• Time in Patent Office: 1,155 Days
• Field of Search: 435/395, 623/23.72
• US Class Current: 435/395
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: C12N 2533/40 Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 5/0653 Adipocytes; Adipose tissue

Abstract

The field of the present invention relates to a novel method for producing tissue or organ in a mammal by implanting in vivo a novel three dimensional biodegradable scaffold. The novel three dimensional biodegradable scaffolds overcome the barrier of developing large organs with tissue engineering.

Claims

1. An implant comprising:

a three dimensional scaffold shell having an interior and an exterior; and

a tissue matrix deposited in the interior of said scaffold shell comprising multiple folds of vascularized omentum in combination with excised fat tissue inserted between said folds of said omentum, wherein said scaffold is comprised of a material that is different from said tissue matrix.

2. An implant as recited in claim 1, wherein said implant is a breast implant.

3. An implant as recited in claim 1, wherein said fat tissue is present as a mixture of fat cells harvested from an organ and a nutrient.

4. An implant as recited in claim 1, wherein said scaffold is comprised of a polymer.

5. A method of generating an implant comprising a three dimensional scaffold shell containing a tissue matrix within its interior, the method comprising:

providing a cell mixture comprising cells harvested from an organ, wherein the cells are immunocompatible with a subject;

identifying a growth site in the subject comprising a vascular tissue having an arterial flow and a venous flow and a capability of growing the cell mixture;

introducing a three dimensional scaffold into said growth site of said subject by placing said vascular tissue present in the growth site into the interior of said three dimensional scaffold shell, wherein the arterial and venous flow between said vascular tissue and said subject remain intact when said vascular tissue is placed into the interior of said scaffold shell;

introducing the cell mixture into said interior of said scaffold shell, wherein the cell mixture associates with said vascular tissue in the interior of said scaffold shell;

allowing said scaffold shell comprising said vascularized tissue and said cell mixture to incubate in said growth site to form an implant comprising said three dimensional scaffold shell comprising a tissue matrix within its interior; and

removing said implant from said growth site.

6. A method according to claim 5, wherein said organ is a breast.

7. A method according to claim 5, wherein said vascularized tissue comprises omentum of a said patient.

8. A method according to claim 5, wherein said cell mixture comprises a mixture of fat cells and a nutrient.

9. A method according to claim 5, wherein the growth site is present in a region of the subject that is selected from the group consisting of the mid-abdomen region, an inguinal region, an axillary region, a medial thigh region, and the substernal region.

10. A method according to claim 5, wherein the three dimensional scaffold shell is bioresorbable.