Ion trapping

• US 7,847,243 B2
• Filed: 03/29/2006
• Issued: 12/07/2010
• Est. Priority Date: 03/29/2005
• Status: Active Grant

• Current Assignee: Thermo Finnigan LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Thermo Finnigan LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Jung, Gerhard, Denisov, Eduard V., Malek, Robert, Makarov, Alexander Alekseevich, Lange, Oliver
• Primary Examiner(s): Souw; Bernard E
• Assistant Examiner(s): SMYTH, ANDREW P
• Application Number:
  US11/909,850
• Publication Number:
  US 20080156984A1
• Time in Patent Office: 1,714 Days
• Field of Search: 250281-300
• US Class Current: 250/288
• CPC Class Codes: H01J 49/004 Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn
• CPC Class Codes: H01J 49/423 with radial ejection
• CPC Class Codes: H01J 49/425 with a logarithmic radial electric potential, e.g. orbitraps
• CPC Class Codes: H01J 49/4295 Storage methods

Abstract

This invention relates to a method of trapping ions and to an ion trapping assembly. In particular, the present invention has application in gas-assisted trapping of ions in an ion trap prior to a mass analysis of the ions in a mass spectrometer. The invention provides a method of trapping ions in a target ion trap of an ion trapping assembly that comprises a series of volumes arranged such that ions can traverse from one volume to the next, the volumes including the target ion trap, whereby ions are allowed to pass repeatedly through the volumes such that they also pass into and out from the target ion trap without being trapped. Potentials may be used to reflect the ions from respective ends of the ion trapping assembly. Optionally, a potential well and/or gas-assisted cooling may be used to cause the ions to settle in the target ion trap.

Claims

1. A method of trapping ions in a target ion trap comprising:

introducing ions into an ion trapping assembly comprising a series of volumes arranged such that ions can traverse from one volume to the next, the volumes including the target ion trap;

allowing the ions to pass into and out from the target ion trap without being trapped; and

guiding the ions such that they pass into the target ion trap for a second time.

2. The method of claim 1, comprising reflecting the ions such that they pass into the target ion trap for the second time.

3. The method of claim 2, further comprising allowing the ions that have passed into the target ion trap for a second time to pass out from the target ion trap without being trapped, and subsequently directing the ions to pass into the target ion trap for a third time.

4. The method of claim 3, comprising placing a first potential at one end of the ion trapping assembly and placing a second potential at the other end of the ion trapping assembly, thereby causing the ions to reflect at either end and so to traverse the target ion trap repeatedly.

5. The method of claim 1, further comprising introducing a gas into at least one of the volumes thereby causing gas-assisted trapping of the ions.

6. The method of claim 5, comprising introducing gas or gases into the target ion trap and an adjacent volume such that the pressure in the target ion trap is lower than in the adjacent volume.

7. The method of claim 1, further comprising applying RF potentials to the ion trapping assembly to produce pseudo-potentials for trapping ions.

8. The method of claim 7, comprising applying RF potentials suitable for trapping both positive and negative ions simultaneously.

9. The method of claim 1, comprising filling with gas a set of volumes including at least the target ion trap, such that the product of the average pressure in the gas-filled volumes and the length of the gas-filled volumes is less than 0.5 Torr-mm.

10. The method of claim 1, comprising filling with gas a set of volumes including at least the target ion trap to a pressure in the range 0.1 mTorr to 10 mTorr.

11. The method of claim 1, further comprising trapping ions in an ion store before releasing ions from the ion store into the ion trapping assembly.

12. The method of claim 11, wherein the step of trapping ions in an ion store before releasing ions from the ion store into the ion trapping assembly includes:

trapping a first group of ions in the ion store;

releasing the first group of ions from the ion store into the ion trapping assembly;

after releasing the first group of ions, trapping a second group of ions in the ion store and releasing the second group of ions into the ion trapping assembly; and

accumulating an ion population including the first and second groups of ions in the target ion trap.

13. The method of claim 1, wherein the ion trapping assembly has a longitudinal axis corresponding broadly to the ions'"'"' motion backwards and forwards through the series of volumes and the method further comprising ejecting ions trapped in the target ion trap substantially orthogonally from the target ion trap.

14. The method of claim 1, wherein the target ion trap comprises first and second volumes of the series of volumes, the method comprising:

applying potentials to the ion trapping assembly such that the potential rises at either end of the target ion trap thereby forming a potential well, and such that potential barriers are formed at either end of the ion trapping assembly;

introducing ions into the ion trapping assembly where they are subsequently reflected by the potential barriers at either end of the ion trapping assembly, thereby traversing the target ion trap repeatedly while they lose energy eventually to settle in the target ion trap; and

subsequently to apply a potential to act between the first and second volumes thereby to split the ions that have settled in the first and second volumes into two groups, one being trapped in the first volume and the other being trapped in the second volume.

15. The method of claim 14, further comprising determining the number of ions in the first volume and using this determination to estimate the number of ions in the second volume.

16. An ion trapping assembly comprising:

a series of volumes arranged such that ions can traverse from one volume to the next, wherein at least one of the volumes are adapted to be filled with gas, and wherein the series of volumes include an axially elongated target ion trap having an entrance end positioned to receive ions from an ion source and an exit end axially opposed to the entrance end;

electrodes arranged to carry potentials; and

a controller configured to set potentials on the electrodes such that (i) the potential rises at either end of the target ion trap, thereby forming a potential well in the target ion trap, (ii) the one or more volumes adapted to be filled with gas positioned adjacent the exit end of the target ion trap are at a higher potential than the target ion trap, and (iii) potential barriers are formed at either end of the ion trapping assembly.

17. The ion trapping assembly of claim 16, comprising an ion reflector corresponding to one of the volumes located adjacent to the target ion trap.

18. The ion trapping assembly of claim 16, wherein the controller is configured to set potentials to produce pseudo-potentials for trapping ions.

19. The ion trapping assembly of claim 16, further comprising a gas supply operable to introduce a gas into at least one of the volumes.

20. The ion trapping assembly of claim 16, wherein the target ion trap comprises first and second volumes of the series of volumes, and the controller is configured to allow a delay for ions to settle in the potential well of the target ion trap and then to set a potential to act between the first and second volumes, thereby forming two potential wells, one in each of the first and second volumes.

21. The ion trapping assembly of claim 16, further comprising an ion source and an ion store positioned downstream in the ion path of the ion source and upstream in the ion path of the series of volumes, and wherein the controller is configured to set potentials on the ion store to trap ions produced by the ion source and then to release trapped ions into the series of volumes.

22. The ion trapping assembly of claim 16, wherein the controller is configured to trap ions in the ion store repeatedly, each time releasing the ion packet into the ion trapping assembly thereby accumulating multiple ion packets in the target ion trap.

23. The ion trapping assembly of claim 16, wherein the ion trapping assembly has a longitudinal axis corresponding broadly to the ions motion backwards and forwards through the series of volumes and the controller is configured to eject ions trapped in the target ion trap substantially orthogonally from the target ion trap.