Analytical tool

• US 7,850,909 B2
• Filed: 09/25/2003
• Issued: 12/14/2010
• Est. Priority Date: 09/26/2002
• Status: Active Grant

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Noda, Yuichiro, Kitamura, Shigeru, Taguchi, Takayuki
• Primary Examiner(s): SIEFKE, SAMUEL P
• Application Number:
  US10/529,120
• Publication Number:
  US 20060045799A1
• Time in Patent Office: 2,637 Days
• Field of Search: 422/50, 422/68.1, 422/73, 422/99, 422/57
• US Class Current: 422/403
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: G01N 1/4077 by other techniques involving separation of suspended solids
• CPC Class Codes: G01N 21/07 Centrifugal type cuvettes G01N21/09 takes precedence
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes
• CPC Class Codes: G01N 35/1065 Multiple transfer devices

Abstract

An analyzing tool (Y) has a liquid-introducing opening (61), one or more flow passages (51) through which a sample liquid introduced from the liquid-introducing opening (61) is moved, and a separation film (8) for filtrating the sample liquid supplied to the liquid-introducing opening (61) and then introducing the liquid filtrated to the one or more flow passages (51). The analyzing tool (Y) is structured such that a liquid sample is filtrated by being advanced in the thickness direction of the separation film (8). The flow passage (51) is structured such that the sample liquid is moved by, for example, a capillary phenomenon.

Claims

1. An analytical tool comprising:

a liquid introduction port,

at least one flow path for moving a sample liquid introduced through the liquid introduction port,

a reaction chamber communicating with said at least one flow path, and

a separation film disposed below the liquid introduction port and above said at least one flow path for filtering the sample liquid supplied to the liquid introduction port before introducing the sample liquid to said at least one flow path;

wherein the sample liquid is caused to move through the separation film in a thickness direction of the separation film for filtration;

wherein a branching flow path branches from said at least one flow path at a branching position upstream from and close to the reaction chamber;

wherein the branching flow path communicates with a first gas discharge port closed by a first seal film that is openable by needle insertion for supplying the sample liquid from the liquid introduction port to the branching position; and

wherein the reaction chamber communicates with a second gas discharge port closed by a second seal film that is openable by needle insertion for supplying the sample liquid beyond the branching position to the reaction chamber.

2. The analytical tool according to claim 1, wherein said at least one flow path moves the sample liquid by capillary action.

3. The analytical tool according to claim 1, wherein the sample liquid comprises blood, and

wherein the separation film separates blood cells from the blood.

4. The analytical tool according to claim 3, wherein the separation film comprises a porous film having a minimum pore size of 0.1˜

3.0 μ

m.

5. The analytical tool according to claim 1, wherein said at least one flow path extends in a plane that is parallel to the separation film.

6. The analytical tool according to claim 5, further comprising a liquid receiving portion for retaining the sample liquid passed through the separation film, the liquid receiving portion communicating with the liquid introduction port and said at least one flow path, and

wherein the separation film is spaced from a bottom surface of the liquid receiving portion.

7. The analytical tool according to claim 6, further comprising:

a substrate in which the liquid receiving portion and said at least one flow path are formed;

a cover in which the liquid introduction port, the first discharge port and the second discharge port are formed; and

an adhesive layer interposed between the substrate and the cover, the adhesive layer including a through-hole for fitting the separation film.

8. The analytical tool according to claim 6, wherein said at least one flow path extends radially from the liquid receiving portion.

9. The analytical tool according to claim 1, wherein a plurality of flow paths are provided for communicating with a plurality of reaction chambers, respectively, at least two of the plurality of reaction chambers each containing a different reagent;

and

wherein the tool is adapted to measure a plurality of items from a single kind of sample liquid.

10. The analytical tool according to claim 9, wherein the plurality of reaction chambers are arranged on a common circle.

11. The analytical tool according to claim 1, wherein said at least one flow path has a principal, rectangular cross section which has a width of 10 to 500 μ

m and a depth of 5 to 500 μ

m and which satisfies depth/width≧

0.5.

12. The analytical tool according to claim 1, wherein said at least one flow path includes a hydrophilically-treated inner surface.

13. The analytical tool according to claim 12, wherein the inner surface of said at least one flow path is so treated that a contact angle of pure water at the inner surface becomes 0-80 degrees.

14. An analytical tool, comprising:

a liquid introduction port,

at least one flow path for moving a sample liquid introduced through the liquid introduction port,

a reaction chamber communicating with said at least one flow path, and

a separation film disposed below the liquid introduction port and above said at least one flow path for filtering the sample liquid supplied to the liquid introduction port before introducing the sample liquid to said at least one flow path;

wherein the sample liquid is caused to move gravitationally through the separation film in a thickness direction of the separation film for filtration;

wherein a branching flow path branches from said at least one flow path at a branching position upstream from and close to the reaction chamber;

wherein the branching flow path communicates with a first gas discharge port closed by a first seal member which is openable for supplying the sample liquid from the liquid introduction port to the branching position; and

wherein the reaction chamber communicates with a second gas discharge port closed by a second seal member which is openable for supplying the sample liquid beyond the branching position to the reaction chamber.

16. The analytical tool according to claim 14, wherein said at least one flow path extends in a plane that is parallel to the separation film.

17. The analytical tool according to claim 16, further comprising a liquid receiving portion for retaining the sample liquid passed through the separation film, the liquid receiving portion communicating with the liquid introduction port and said at least one flow path, and

wherein the separation film is spaced from a bottom surface of the liquid receiving portion.

18. The analytical tool according to claim 17, further comprising:

a substrate in which the liquid receiving portion and said at least one flow path are formed;

a cover in which the liquid introduction port, the first discharge port and the second discharge port are formed; and

an adhesive layer interposed between the substrate and the cover, the adhesive layer including a through-hole for fitting the separation film.

19. The analytical tool according to claim 18, wherein said at least one flow path extends radially from the liquid receiving portion.

20. The analytical tool according to claim 18, wherein a plurality of flow paths are provided for communicating with a plurality of reaction chambers, respectively;

and

wherein the plurality of flow paths communicate with the second discharge port via a common flow path formed in the cover.

15. An analytical apparatus comprising an analytical tool and a hole-making mechanism, the analytical tool comprising:

a liquid introduction port,

at least one flow path for moving a sample liquid introduced through the liquid introduction port,

a reaction chamber communicating with said at least one flow path, and

a separation film for filtering the sample liquid supplied to the liquid introduction port before introducing the sample liquid to said at least one flow path;

wherein the sample liquid is caused to move through the separation film in a thickness direction of the separation film for filtration;

wherein a branching flow path branches from said at least one flow path at a branching position upstream from and close to the reaction chamber;

wherein the branching flow path communicates with a first gas discharge port closed by a first seal film which is openable by needle insertion for supplying the sample liquid from the liquid introduction port to the branching position;

wherein the reaction chamber communicates with a second gas discharge port closed by a second seal film which is openable by needle insertion for supplying the sample liquid beyond the branching position to the reaction chamber; and

wherein the hole-making mechanism comprises a first hole-making needle for perforating the first seal film, and a second hole-making needle for perforating the second seal film.