Compound screening using cardiomyocytes
First Claim
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1. A method of screening a compound for an effect on a cardiomyocyte in cell culture, comprising:
- a) contacting the cell culture with the compound; and
b) observing a change in a cardiomyocyte in the cell culture compared to a cardiomyocyte in an untreated cell culture andc) determining a difference between the treated and untreated cardiomyocyte in the respective cell cultures,wherein the cell culture comprises 5% or more cardiomyocytes that are at least 99.6% genetically identical to an established line of non-malignant cells which express SSEA3, SSEA4, Tra-1-60 and Tra-1-81.
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Abstract
This invention provides populations human cells of the cardiomyocyte lineage. The cells are obtained by causing cultures of pluripotent stem cells to differentiate in vitro, and then harvesting cells with certain phenotypic features. Differentiated cells bear cell surface and morphologic markers characteristic of cardiomyocytes, and a proportion of them undergo spontaneous periodic contraction. Highly enriched populations of cardiomyocytes and their replicating precursors can be obtained, suitable for use in a variety of applications, such as drug screening and therapy for cardiac disease.
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Citations
24 Claims
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1. A method of screening a compound for an effect on a cardiomyocyte in cell culture, comprising:
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a) contacting the cell culture with the compound; and b) observing a change in a cardiomyocyte in the cell culture compared to a cardiomyocyte in an untreated cell culture and c) determining a difference between the treated and untreated cardiomyocyte in the respective cell cultures, wherein the cell culture comprises 5% or more cardiomyocytes that are at least 99.6% genetically identical to an established line of non-malignant cells which express SSEA3, SSEA4, Tra-1-60 and Tra-1-81. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method of screening a compound for an effect on a cell in cell culture, comprising:
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a) contacting the cell culture with a compound; and b) observing a change in a myosin heavy chain expressing cell in the cell culture compared to a myosin heavy chain expressing cell in an untreated cell culture and c) determining a difference between the treated and untreated myosin heavy chain expressing cell in the respective cell cultures, wherein the cell culture comprises 5% or more cells that express myosin heavy chain and are at least 99.6% genetically identical to an established line of non-malignant cells which express SSEA3, SSEA4, Tra-1-60 and Tra-1-81. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification