Template methods and devices for preparing sample arrays

• US 7,854,899 B2
• Filed: 08/26/2004
• Issued: 12/21/2010
• Est. Priority Date: 08/26/2004
• Status: Active Grant

• Current Assignee: United States Department Of Health And Human Services
• Original Assignee: United States Department Of Health And Human Services
• Inventors: Hewitt, Stephen M.
• Primary Examiner(s): LEVKOVICH, NATALIA A
• Application Number:
  US10/928,656
• Publication Number:
  US 20060046282A1
• Time in Patent Office: 2,308 Days
• Field of Search: 422/104, 422/102, 422/99
• US Class Current: 422/505
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00313 the reactor vessels being formed by arrays of wells in blocks
• CPC Class Codes: B01J 2219/00373 Hollow needles
• CPC Class Codes: B01J 2219/0043 for direct application of reagents, e.g. through openings in a shutter
• CPC Class Codes: B01J 2219/0074 Biological products
• CPC Class Codes: B01J 2219/00743 Cells
• CPC Class Codes: C40B 60/14 for creating libraries

Abstract

A method for preparing a microarray that includes placing at least one template over a first surface of the recipient block, wherein the template defines an array of openings and the recipient block has a plurality of receptacle holes, such that the array of openings are aligned with the plurality of receptacle holes. A needle or punch that contains a sample is inserted through the openings of the template. The sample then is inserted into the receptacle hole in the recipient block. A device is also disclosed that includes a platform defining (i) a first surface, and (ii) a first region configured to retain at least one recipient block; and a raised template defining an array of openings, secured to the first surface of the platform and positioned above the first region configured to retain the recipient block.

Claims

1. A device for preparing an array of samples, comprising:

at least one tissue microarray recipient block having a thickness, wherein the recipient block comprises an embedding medium and includes a plurality of receptacle holes in the embedding medium that do not extend through the entire thickness of the recipient block;

a platform defining (i) a first surface, and (ii) a first cavity formed in the first surface and configured to retain the at least one recipient block;

a raised template defining an array of openings, secured to the first surface of the platform and positioned above the first cavity configured to retain the recipient block and a second template positioned over the raised template, the second template defining an array of openings, the second template openings being aligned with the openings of the raised template, each of said templates being supported by at least two support elements such that the second template clamps the raised template to the first surface and such that the raised template and the first cavity form a slot adapted to receive the recipient block, wherein the template openings are adapted to guide a needle or punch into at least one of the receptacle holes.

2. The device of claim 1, wherein the openings of the template are circular and have a diameter of about 1.5 mm to about 4.0 mm, and the openings of the template are spaced apart from each other by about 1.5 mm to about 4.0 mm.

3. The device of claim 1, wherein the raised template includes from 10 to 1000 openings.

4. The device of claim 1, wherein the embedding medium is sliceable by a microtome.

5. The device of claim 1, wherein the raised template is permanently secured to the first surface of the platform.

6. The device of claim 1, wherein the first surface also includes a region that retains at least one donor block.

7. The device of claim 1, wherein the first cavity defines a floor and sidewalls.

8. The device of claim 1, wherein the raised template is a metal template.

9. The device of claim 1, wherein the array of openings are aligned with the plurality of receptacle holes.

10. The device of claim 1, wherein the receptacle holes have a depth of 2 to 20 mm.

11. The device of claim 1, wherein the recipient block comprises a cryoarray.

12. The device of claim 1, wherein the embedding medium comprises a rigid material selected from paraffin, gelatin, a plastic, or a cryoarray medium.

13. The device of claim 1, wherein the samples are solid samples.

14. The device of claim 1, wherein the samples are biological tissue samples.

15. The device of claim 1, wherein the samples are frozen samples.

16. A device for preparing an array of samples, comprising:

a platform defining (i) a first surface, (ii) a first cavity formed in the first surface and retaining at least one tissue microarray recipient block having a thickness, wherein the recipient block comprises an embedding medium and includes a plurality of receptacle holes in the embedding medium that do not extend through the entire thickness of the recipient block, and (iii) a region located on the first surface and configured to retain at least one tissue donor block;

a raised template defining an array of openings, secured to the first surface of the platform and positioned above the first cavity configured to retain the recipient block and a second template positioned over the raised template, the second template defining an array of openings;

the second template openings being aligned with the openings of the raised template;

each of said templates being supported by at least two support elements such that the second template clamps the raised template to the first surface and such that the raised template and the first cavity form a slot adapted to receive the recipient block, wherein the template openings are adapted to guide a needle or punch into at least one of the receptacle holes.