Plant acyltransferases specific for long-chained, multiply unsaturated fatty acids
First Claim
1. A process for the production of polyunsaturated fatty acids in an organism comprising(a) introducing, into the organism, at least one nucleic acid comprising the sequence shown in SEQ ID NO:
- 16 which codes for a polypeptide with lysophosphatidic acid acyltransferase activity;
or(b) introducing, into the organism, at least one nucleic acid comprising a nucleic acid sequence having at least 95% identity with the sequence of SEQ ID NO;
16, wherein the nucleic acid codes for a polypeptide with lysophosphatidic acid acyltransferase activity, or(c) introducing, into the organism, at least one nucleic acid which codes for a polypeptide comprising the amino acid sequence shown in SEQ ID NO;
17 or comprising an amino acid sequence having at least 95% identity with SEQ ID NO;
17 and has lysophosphatidic acid acyltransferase activity, and(d) culturing and harvesting the organismwherein the organism is a microorganism, yeast, or a plant.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates to a process for the production of long-chain polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids which code for polypeptides with acyl transferase activity. These nucleic acid sequences, if appropriate together with further nucleic acid sequences which code for polypeptides of the fatty acid or lipid metabolism biosynthesis, can advantageously be expressed in the organism. Furthermore, the invention relates to a method for the production of oils and/or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids.
The invention furthermore relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms comprising the nucleic acid sequences according to the invention, vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors.
A further part of the invention relates to oils, lipids and/or fatty acids produced by the process according to the invention and to their use.
-
Citations
21 Claims
-
1. A process for the production of polyunsaturated fatty acids in an organism comprising
(a) introducing, into the organism, at least one nucleic acid comprising the sequence shown in SEQ ID NO: - 16 which codes for a polypeptide with lysophosphatidic acid acyltransferase activity;
or(b) introducing, into the organism, at least one nucleic acid comprising a nucleic acid sequence having at least 95% identity with the sequence of SEQ ID NO;
16, wherein the nucleic acid codes for a polypeptide with lysophosphatidic acid acyltransferase activity, or(c) introducing, into the organism, at least one nucleic acid which codes for a polypeptide comprising the amino acid sequence shown in SEQ ID NO;
17 or comprising an amino acid sequence having at least 95% identity with SEQ ID NO;
17 and has lysophosphatidic acid acyltransferase activity, and(d) culturing and harvesting the organism wherein the organism is a microorganism, yeast, or a plant. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
- 16 which codes for a polypeptide with lysophosphatidic acid acyltransferase activity;
-
10. An isolated nucleic acid comprising a nucleic acid sequence selected from the group consisting of:
-
(a) the nucleic acid sequence shown in SEQ ID NO;
16,(b) a nucleic acid sequence having at least 95% identity with the sequence of SEQ ID NO;
16 and coding for a polypeptide having lysophosphatidic acid acyltransferase activity,(c) a nucleic acid sequence encoding a polypeptide comprising the amino acid sequence shown in SEQ ID NO;
17, and(d) a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence having at least 95% identity with the sequence of SEQ ID NO;
17 and having lysophosphatidic acid acyltransferase activity. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
-
Specification