Fluid dispenser for fluid in assay

• US 7,858,041 B2
• Filed: 09/05/2006
• Issued: 12/28/2010
• Est. Priority Date: 09/02/2005
• Status: Expired due to Fees

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Inventors: Fujikura, Tatsuo, Takai, Shinya, Muraishi, Katsuaki
• Primary Examiner(s): Gordon; Brian R
• Application Number:
  US11/514,885
• Publication Number:
  US 20070053797A1
• Time in Patent Office: 1,575 Days
• Field of Search: 422/100, 422 63- 681
• US Class Current: 422/511
• CPC Class Codes: B01L 2200/022 Variable spacings
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/021 Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: G01N 35/1095 for supplying the samples to flow-through analysers for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00

Abstract

A fluid dispenser for surface plasmon resonance (SPR) assay apparatus includes a first pipette device on a first pipette head, and a second pipette device on a second pipette head. In a standby state, the first pipette head is set in an upper position, a coil spring and a stopper set the second pipette head together with the first pipette head, and locate the second pipette head in a high position. While the first and second pipette devices are in an aspirating position, a driving mechanism sets the first pipette head in a lower position. A blocking mechanism sets the second pipette head in a high position against the coil spring. First, third and fifth pipette tips is positioned lower than second, fourth and sixth pipette tips, to access a well. The first to sixth pipette tips, while in an assay position, are equally lowered.

Claims

1. An assay apparatus comprising:

a multi well plate, comprising a plurality of wells;

a sample contained in said plurality of wells;

a sensor unit comprising a flow channel and a sensing surface disposed in said flow channel, said sensing surface configured to assay reaction of said sample flowing through said flow channel;

stopper rods;

an assay unit configured to position said sensor unit for assay; and

a fluid dispenser comprising;

a dispensing head, comprising a first head and a second head, movable between an aspirating position and an assay position,

wherein the dispensing head is configured to aspirate said sample by accessing one of said plurality of wells in said multi well plate in a downward accessing direction, when in said aspirating position; and

wherein the fluid dispenser head is configured to introduce said sample by accessing in a downward accessing direction into said flow channel in said sensor unit, when in said assay position,

wherein said first head supports a first pipette device,

wherein said first pipette device comprises a first pipette tip, and is configured to be set in said well and aspirate said sample, and set in a first orifice of said flow channel to dispense said sample;

wherein said second head supports a second pipette device,

wherein said second pipette device comprises a second pipette tip, and is configured to be set in a second orifice of said flow channel and aspirate fluid in synchronism with dispensation said sample by said first pipette device;

a driving mechanism configured to shift up or down said first head to move between a first position and a second position,

wherein said first position positions said first pipette tip in said well or said first orifice, and said second position positions said first head higher than said first position and said first pipette tip away from said well or said first orifice;

a guide mechanism configured to support said second head movably in a vertical shifting direction of said first head and relatively thereto;

a stopper portion of said first head configured to engage said second head when said second pipette tip is positioned at substantially the same height as said pipette tip of said first pipette device, preventing said second head from moving further downwards;

a biasing mechanism for biasing said second head toward said stopper portion,

wherein said stopper rods are configured to engage said second head when said dispensing head is in said aspirating position, preventing said second head from moving down together with said first head against a bias of said biasing mechanism.

2. The assay apparatus according to claim 1, wherein said second head comprises:

a plurality of lateral sides; and

projecting rods protruding from each of said plurality of lateral sides of said second head and engaging with said stopper rods.

3. The assay apparatus according to claim 1, wherein said guide mechanism comprises:

a slide rail portion formed on said first head; and

a slidable portion formed with said second head engaging with said slide rail portion in a slidable manner.

4. The assay apparatus according to claim 1, wherein said first head comprises a plurality of said first pipette device, and said second head comprises a plurality of said second pipette devices.

5. The assay apparatus according to claim 1, further comprising a position sensor configured to detect said first head in said second position.

6. The assay apparatus according to claim 1, further comprising a controller.

7. The assay apparatus according to claim 2, further comprising a controller.

8. The assay apparatus according to claim 3, further comprising a controller.

9. The assay apparatus according to claim 4, further comprising a controller.

10. The assay apparatus according to claim 5, further comprising a controller.