Method of combing a nucleic acid

• US 7,858,305 B2
• Filed: 06/30/2006
• Issued: 12/28/2010
• Est. Priority Date: 06/30/2006
• Status: Expired due to Fees

• Current Assignee: Enterprise Science Fund, LLC (Intellectual Ventures LLC)
• Original Assignee: The Invention Science Fund I, L.L.C. (Intellectual Ventures LLC)
• Inventors: Wood, Lowell L. Jr., Hyde, Roderick A.
• Primary Examiner(s): Lam; Ann Y
• Application Number:
  US11/480,820
• Publication Number:
  US 20080003586A1
• Time in Patent Office: 1,642 Days
• Field of Search: 435/4, 435/7.1, 435/7.92, 436/514, 436/518
• US Class Current: 435/6.16
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/0038 Drawing
• CPC Class Codes: B01J 2219/00382 Stamping
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00727 Glycopeptides
• CPC Class Codes: B01J 2219/00734 Lipids
• CPC Class Codes: B01J 2219/00756 Compositions, e.g. coatings, crystals, formulations
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 1/6841 : In situ hybridisation
• CPC Class Codes: C12Q 2565/629 : being a microfluidic device
• CPC Class Codes: H10K 71/191 : characterised by provisions for the orientation or alignment of the layer to be deposited
• CPC Class Codes: H10K 85/761 : Biomolecules or bio-macromolecules, e.g. proteins, chlorophyl, lipids or enzymes

Abstract

Elongated molecules are stretched across a substrate by controlled fluid flow.

Claims

1. A method of applying a nucleic acid to a surface, comprising:

applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor; and

controllably flowing a liquid deposition fluid over the first substrate by applying a cover surface to the deposition fluid in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing further comprises inducing surface charges on at least one of the cover surface and the first substrate.

2. The method of claim 1, wherein inducing surface charges comprises photoinducing surface charges.

3. The method of claim 1, wherein the induced surface charges are static.

4. The method of claim 1, wherein the induced surface charges are dynamic.

5. The method of claim 1, wherein the induced surface charges are spatially patterned.

6. A method of applying a nucleic acid to a surface, comprising:

applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor; and

controllably flowing a liquid deposition fluid over the first substrate by applying a cover surface to the deposition fluid in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing comprises directing fluid flow by controlling hydrophilicity of at least one of the cover surface and the first substrate.

7. A method of applying a nucleic acid to a surface, comprising:

applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor; and

controllably flowing a liquid deposition fluid over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing comprises inducing voracity into the deposition fluid.

8. The method of claim 7, wherein the fluid flow promotes deposition of a nucleic acid molecule in a curve along the substrate.

9. A method of applying a nucleic acid to a surface, comprising:

applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor; and

controllably flowing a liquid deposition fluid over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing comprises directing fluid flow by applying a varying surface tension along the first substrate.

10. A method of applying a nucleic acid to a surface, comprising:

applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor; and

controllably flowing a liquid deposition fluid over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing comprises directing fluid flow by controlling hydrophilicity of the first substrate.

11. The method of claim 10, wherein controlling hydrophilicity of the first substrate comprises dynamically changing the hydrophilicity of the first substrate.

12. A method of applying a nucleic acid to a surface, comprising:

applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor; and

controllably flowing a liquid deposition fluid over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing comprises varying the temperature of the deposition fluid.