Methods and apparatus for conducting multiple measurements on a sample
First Claim
1. A method for assaying for an analyte of interest comprising(a) introducing a sample comprising said analyte into a multi-well plate having a plurality of wells and at least two assay domains located within a single well of said plurality wells, said at least two assay domains including (i) a first domain comprising a first component that binds said analyte, and (ii) a second domain comprising a second component that competes with said analyte;
- (b) conducting in said single well a sandwich assay and a competitive assay, wherein the amount of said analyte is separately quantified in said sandwich assay and said competitive assay; and
(c) comparing the amounts of said analyte identified in step (b) in said sandwich and said competitive assays to determine if a dynamic range of said sandwich assay has been exceeded.
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Accused Products
Abstract
Multiplexed test measurements are conducted using an assay module having a plurality of assay domains. In preferred embodiments, these measurements are conducted in assay modules having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.
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Citations
8 Claims
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1. A method for assaying for an analyte of interest comprising
(a) introducing a sample comprising said analyte into a multi-well plate having a plurality of wells and at least two assay domains located within a single well of said plurality wells, said at least two assay domains including (i) a first domain comprising a first component that binds said analyte, and (ii) a second domain comprising a second component that competes with said analyte; -
(b) conducting in said single well a sandwich assay and a competitive assay, wherein the amount of said analyte is separately quantified in said sandwich assay and said competitive assay; and (c) comparing the amounts of said analyte identified in step (b) in said sandwich and said competitive assays to determine if a dynamic range of said sandwich assay has been exceeded. - View Dependent Claims (2, 3, 4)
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5. A method for assaying for an analyte of interest, the method comprising:
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(a) introducing a sample comprising said analyte into an assay module comprising a plurality of particles including;
(i) a first particle comprising a first assay domain including a first component that binds said analyte, and (ii) a second particle comprising a second assay domain including a second component that competes with said analyte;(b) conducting, simultaneously, in said assay module a sandwich assay and a competitive assay, wherein the amount of said analyte is separately quantified in said sandwich assay and said competitive assay; and (c) comparing the amounts of said analyte identified in step (b) in said sandwich and said competitive assays to determine if a dynamic range of said sandwich assay has been exceeded. - View Dependent Claims (6, 7, 8)
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Specification