Method for detecting binding events using micro-X-ray fluorescence spectrometry

• US 7,858,385 B2
• Filed: 05/16/2001
• Issued: 12/28/2010
• Est. Priority Date: 05/16/2001
• Status: Expired due to Term

• Current Assignee: Icagen, Inc.
• Original Assignee: Los Alamos National Security LLC (Government of the United States of America)
• Inventors: Mann, Grace, Havrilla, George J., Warner, Benjamin P.
• Primary Examiner(s): Gabel; Gailene R
• Assistant Examiner(s): FOSTER, CHRISTINE E
• Application Number:
  US09/859,701
• Publication Number:
  US 20030027129A1
• Time in Patent Office: 3,513 Days
• Field of Search: 436/518, 436/173, 436/172, 435/7.1, 435/174, 435/7.2
• US Class Current: 436/172
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: Y10S 430/167 X-ray
• CPC Class Codes: Y10T 436/16 Phosphorus containing
• CPC Class Codes: Y10T 436/18 Sulfur containing
• CPC Class Codes: Y10T 436/19 Halogen containing

Abstract

Method for detecting binding events using micro-X-ray fluorescence spectrometry. Receptors are exposed to at least one potential binder and arrayed on a substrate support. Each member of the array is exposed to X-ray radiation. The magnitude of a detectable X-ray fluorescence signal for at least one element can be used to determine whether a binding event between a binder and a receptor has occurred, and can provide information related to the extent of binding between the binder and receptor.

Claims

1. A method for detecting a binding event between at least one binder and members of a receptor array, comprising the steps of:

(a) exposing a plurality of bead-supported and nonradioactive receptors to at least one binder having a chemically associated and nonradioactive element detectable by X-ray fluorescence to form at least one bound, bead-supported receptor-binder complex;

wherein the element is selected from the group consisting of P, S, and Br; and

wherein the binder is selected from the group consisting of esters, amines, imines, aldehydes, ketones, amides, ethers, olefins, halogenated organic molecules, antibodies, drugs, hormones, steroids, amino acids, nucleic acids, oligomers, oligonucleotides, oligosaccharides, oligopeptides, polyolefins, polyurethanes, polyesters, polycarbonates, polyamines, polyamides, halogenated polymers, polypeptides, polynucleotides, polysaccharides, anions, complex ions, oxoanions, polyoxoanions, phosphate, organophosphates, sulfate, organosulfates, zirconate, agonists and antagonists for cell membrane receptors, toxins, enzymes, enzyme substrates, cofactors, and antibodies;

(b) washing said bound, bead-supported receptor-binder complex to remove unbound binder from said bound, bead-supported receptor-binder complex;

(c) arraying said bound, bead-supported receptor-binder complex onto a substrate; and

(d) detecting a binding event by exposing said bound, bead-supported receptor-binder complex to X-ray radiation in order to generate an X-ray fluorescence signal from said detectable element in said bound, bead-supported receptor-binder complex.

2. The method of claim 1, wherein the receptor comprises at least one organic compound.

3. The method of claim 1, wherein the receptor comprises at least one oligomer.

4. The method of claim 1, wherein the receptor comprises at least one polymer.

5. The method of claim 1, wherein the receptor is selected from the group consisting of esters, amines, imines, aldehydes, ketones, amides, ethers, olefins, halogenated organic molecules, antibodies, drugs, steroids, amino acids, nucleic acids, oligomers, oligonucleotides, oligosaccharides, oligopeptides, polyolefins, polyurethanes, polyesters, polycarbonates, polyamines, polyamides, halogenated polymers, polypeptides, polynucleotides, polysaccharides, cell membrane receptors, viruses, cells, cellular membranes, and organelles.

6. The method of claim 1, wherein the binder comprises at least one organic molecule.

7. The method of claim 1, wherein the binder comprises at least one oligomer.

8. The method of claim 1, wherein the binder comprises at least one polymer.

9. A method for detecting chemical binding between at least one binder and members of a receptor array, comprising the steps of:

(a) exposing a plurality of bead-supported and nonradioactive receptors to at least one untagged binder having a chemically associated and nonradioactive element detectable by X-ray fluorescence to form at least one bound, bead-supported receptor;

wherein the element is selected from the group consisting of P, S, and Br; and

wherein the binder is selected from the group consisting of esters, amines, imines, aldehydes, ketones, amides, ethers, olefins, halogenated organic molecules, antibodies, drugs, hormones, steroids, amino acids, nucleic acids, oligomers, oligonucleotides, oligosaccharides, oligopeptides, polyolefins, polyurethanes, polyesters, polycarbonates, polyamines, polyamides, halogenated polymers, polypeptides, polynucleotides, polysaccharides, anions, complex ions, oxoanions, polyoxoanions, phosphate, organophosphates, sulfate, organosulfates, zirconate, agonists and antagonists for cell membrane receptors, toxins, enzymes, enzyme substrates, cofactors, and antibodies;

(b) washing said bound, bead-supported receptor;

(c) arraying said bound, bead-supported receptor onto a substrate; and

(d) detecting an X-ray fluorescence signal generated by said detectable element in said bound, bead-supported receptor.

10. The method of claim 9, wherein the receptor comprises at least one organic compound.

11. The method of claim 9, wherein the receptor comprises at least one oligomer.

12. The method of claim 9, wherein the receptor comprises at least one polymer.

13. The method of claim 9, wherein the receptor is selected from the group consisting of esters, amines, imines, aldehydes, ketones, amides, ethers, olefins, halogenated organic molecules, antibodies, drugs, steroids, amino acids, nucleic acids, polyurethanes, polyesters, polycarbonates, polyamines, polyamides, halogenated polymers, polypeptides, polynucleotides, polysaccharides, cell membrane receptors, viruses, cells, cellular membranes, and organelles.

14. The method of claim 9, wherein the binder comprises at least one organic molecule.

15. The method of claim 9, wherein the binder comprises at least one oligomer.

16. The method of claim 9, wherein the binder comprises at least one polymer.