Process for purifying glycopeptide phosphonate derivatives

• US 7,858,583 B2
• Filed: 11/17/2008
• Issued: 12/28/2010
• Est. Priority Date: 08/24/2001
• Status: Expired due to Fees

• Current Assignee: Cumberland Pharmaceuticals Inc.
• Original Assignee: Theravance, Inc
• Inventors: Schmidt, Donald E. Jr., Sganga, Jeanmarie Donovan
• Primary Examiner(s): Tate; Christopher R.
• Assistant Examiner(s): Teller; Roy
• Application Number:
  US12/272,554
• Publication Number:
  US 20090215673A1
• Time in Patent Office: 771 Days
• Field of Search: None
• US Class Current: 514/3.1
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: C07K 9/00 Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof
• CPC Class Codes: C07K 9/008 directly attached to a hetero atom of the saccharide radical, e.g. actaplanin, avoparcin, ristomycin, vancomycin

Abstract

Disclosed are methods of purifying glycopeptides that are substituted with one or more substituents each comprising one or more phosphono groups that are useful as antibacterial agents. The methods include contacting a solution of the glycopeptide derivatives with a polystyrene-containing resin, eluting the resin with an aqueous solution, and isolating the purified glycopeptide derivative.

Claims

1. A composition comprising:

(a) a compound of the formula;

2. The composition of claim 1, wherein the polar organic solvent is ethanol.

3. The composition of claim 1, wherein the polar organic solvent is acetonitrile.

4. The composition of claim 1, wherein the acidified aqueous solution contains acetic acid.

5. The composition of claim 1, wherein the acidified aqueous solution contains hydrochloric acid.

6. The composition of claim 1, wherein the polystyrene divinyl benzene resin has a pore size of from about 50 Å

to about 1000 Å

.

7. The composition of claim 1, wherein the polystyrene divinyl benzene resin has a particle bead size in the range of between about 20 μ

m to about 800 μ

m.